{"refrec":{"BRefID":367480,"RR":"<b>Blaschke, V.; Berten, A.; Sprenger, H.; Zagon, J.; Winkel, M.</b> (2023). Filling analytical gaps in allergen detection-real-time PCR for the detection of commercially relevant cephalopods and gastropods in food. <i>J. Agric. Food Chem. 71(31)</i>: 12029-12042. <a href=\"https://dx.doi.org/10.1021/acs.jafc.2c08966\" target=\"_blank\">https://dx.doi.org/10.1021/acs.jafc.2c08966</a>","BEntID":365205,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Agric. Food Chem. 71(31)</i>: 12029-12042. <a href=\"https://dx.doi.org/10.1021/acs.jafc.2c08966\" target=\"_blank\">https://dx.doi.org/10.1021/acs.jafc.2c08966</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Blaschke, V.; Berten, A.; Sprenger, H.; Zagon, J.; Winkel, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Blaschke, V. <i>et al.</i>","Englishabstract":"Mollusks belong to the group of shellfish, which are considered to be among the elicitors of severe food allergies worldwide. In recent years, numerous PCR detection methods have been developed for other shellfish such as crustaceans. However, cephalopods and gastropods were not considered in the development of these shellfish detection systems. In this study, we have developed highly specific real-time PCR methods for the comprehensive detection of all commercially relevant cephalopod species and the gastropod families Helicidae, Buccinidae, and Muricidae in food matrices. In total, we cross-tested over 100 animal and plant species to show the specificity of our systems. The limit of detection (LOD<sub>12</sub>) was set at 1 pg of cephalopod and gastropod DNA or 10 ppm (mg/kg) spiked in a vegetarian food product. 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