Document of bibliographic reference 367480

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Filling analytical gaps in allergen detection-real-time PCR for the detection of commercially relevant cephalopods and gastropods in food

Abstract

Mollusks belong to the group of shellfish, which are considered to be among the elicitors of severe food allergies worldwide. In recent years, numerous PCR detection methods have been developed for other shellfish such as crustaceans. However, cephalopods and gastropods were not considered in the development of these shellfish detection systems. In this study, we have developed highly specific real-time PCR methods for the comprehensive detection of all commercially relevant cephalopod species and the gastropod families Helicidae, Buccinidae, and Muricidae in food matrices. In total, we cross-tested over 100 animal and plant species to show the specificity of our systems. The limit of detection (LOD₁₂) was set at 1 pg of cephalopod and gastropod DNA or 10 ppm (mg/kg) spiked in a vegetarian food product. The robustness of the protocol was confirmed by testing multiple parameters while cooking and autoclaving of samples ensured the practical applicability of the systems.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:001037500400001

Bibliographic citation

Blaschke, V.; Berten, A.; Sprenger, H.; Zagon, J.; Winkel, M. (2023). Filling analytical gaps in allergen detection-real-time PCR for the detection of commercially relevant cephalopods and gastropods in food. J. Agric. Food Chem. 71(31): 12029-12042. https://dx.doi.org/10.1021/acs.jafc.2c08966

Topic

Marine

Is peer reviewed

true