Document of bibliographic reference 367481

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Molecular characterization of sporocysts and Cercariae (Digenea: Bucephalidae) infecting the eastern oyster Crassostrea virginica from Virginia

Abstract

The identity of a larval bucephalid infecting the eastern oyster, Crassostrea virginica (Gmelin, 1791), from a tidal river in Virginia is investigated using ribosomal deoxyribonucleic acid (rDNA). The internal transcribed spacer (ITS1, 5.8S, ITS2) region and a portion of the 28S rDNA were isolated from genomic DNA from sporocysts containing cercariae and compared with sequences from GenBank and from our previous collections of possibly related bucephalids. The studied larval bucephalid was 100% identical at the ITS1, 5.8S, and partial 28S rDNA sequences with Prosorhynchoides paralichthydis (Corkum, 1961) Curran and Overstreet, 2009; however, it differed from P. paralichthydis by 6 bases plus 3 deletions in the ITS2 region. This level of variation at the ITS2 region has been demonstrated among some Indo-Pacific species of Prosorhynchoides Dollfus, 1929, suggesting that the larval bucephalid represents an unidentified or innominate species of Prosorhynchoides that is closely related to P. paralichthydis.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:001029144700007

Bibliographic citation

Curran, S.S.; Gonzales, R.D.; Bullard, S.A. (2023). Molecular characterization of sporocysts and Cercariae (Digenea: Bucephalidae) infecting the eastern oyster Crassostrea virginica from Virginia. J. Parasitol. 109(3): 259-263. https://dx.doi.org/10.1645/22-100

Topic

Marine

Is peer reviewed

true