{"refrec":{"BRefID":368021,"RR":"<b>Zattara, E.E.; Fernandez-Alvarez, F.A.</b> (2022). Collecting and culturing <i>Lineus sanguineus</i> to study Nemertea WBR, <b><i>in</i></b>: Blanchoud, S. <i>et al.</i> <i>Whole-body regeneration: Methods and protocols . Methods in Molecular Biology,</i> 2450: pp. 227-243. <a href=\"https://dx.doi.org/10.1007/978-1-0716-2172-1_12\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-0716-2172-1_12</a>","BEntID":365747,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Blanchoud, S. <i>et al.</i> <i>Whole-body regeneration: Methods and protocols. Methods in Molecular Biology,</i> 2450: pp. 227-243. <a href=\"https://dx.doi.org/10.1007/978-1-0716-2172-1_12\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-0716-2172-1_12</a>","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zattara, E.E.; Fernandez-Alvarez, F.A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zattara, E.E.; Fernandez-Alvarez, F.A.","Englishabstract":"Whole-body regeneration, the ability to reconstruct complete individuals from small fragments, is rare among ribbon worms (phylum Nemertea) but present in the pilidiophoran species <i>Lineus sanguineus</i>. This species can regenerate complete individuals from a tiny midbody section, and even from a quarter of a piece, provided it retains a fragment of a lateral nerve cord. While a few other unrelated species of ribbon worms are also excellent regenerators, <i>L. sanguineus</i> is unique in having evolved its regenerative abilities quite recently and thus offers an exceptional opportunity to gain insight into the evolutionary mechanisms of regeneration enhancement. Interestingly, both its sister species <i>Lineus lacteus</i> and <i>Lineus pseudolacteus,</i> a third species derived from the recent hybridization of the other two, differ in their regeneration abilities: while <i>L. lacteus</i> is uncapable of regenerating a lost head, <i>L. pseudolacteus</i> is capable of anterior regeneration, albeit at a slower rate than <i>L. sanguineus</i>. <i>L. sanguineus</i> has a worldwide distribution in temperate shores of both hemispheres, is readily found at intertidal habitats, and can survive, feed and be bred through asexual replication with minimal effort in laboratory settings. All the above make this species a superb candidate for studies of regenerative biology. In this chapter, we present protocols to collect, identify and breed <i>L. sanguineus</i> to study the extraordinary whole-body regeneration abilities found in this species.","AbstractOtherLang":null,"BibLvlCode":"AMS","StandardTitle":"Collecting and culturing <i>Lineus sanguineus</i> to study Nemertea WBR","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Intertidal, Invertebrate rearing, Spiralia","OtherDescriptors":null,"Notes":null,"AnaPub":2022,"MonPub":null,"DateUpdate":"2023-10-16","DateCreate":"2023-10-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1007/978-1-0716-2172-1_12"},"refs":null,"anarec":{"AnaID":368021,"PubliDate":2022,"Pagination":"227-243","XtraPublOfAnaID":null,"ISBN":null,"Volume":"2450","Issue":null,"BRefMon":368020,"BRefMonRR":"<b>Blanchoud, S.; Galliot, B. (Ed.)</b> (2022). Whole-body regeneration: Methods and protocols. <i>Methods in Molecular Biology</i>, 2450. Humana Press: New York. XVII, 685 pp. <a href=\"https://dx.doi.org/10.1007/978-1-0716-2172-1\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-0716-2172-1</a>","BRefXtra":null,"BRefXtraRR":null,"SerBRefID":294813,"SerRR":"Methods in Molecular Biology. Humana Press.  ISSN 1064-3745","StandardTitleSer":"Methods in Molecular Biology","ISSN":"1064-3745","AbbrevSer":null,"StandardTitleMon":"Whole-body regeneration: Methods and protocols","StartPage":227,"Pages":17,"ToPubliDate":null,"BRefBibLvlCode":"MS","SerNotes":null,"AutString":"Blanchoud, S.; Galliot, B. 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