{"refrec":{"BRefID":368700,"RR":"<b>Le Lay, C.; Hamm, J.N.; Williams, T.J.; Shi, M.; Cavicchioli, R.; Holmes, E.C.</b> (2023). Viral community composition of hypersaline lakes. <i>Advances in Virus Research 9(2)</i>. <a href=\"https://dx.doi.org/10.1093/ve/vead057\" target=\"_blank\">https://dx.doi.org/10.1093/ve/vead057</a>","BEntID":366426,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Advances in Virus Research 9(2)</i>. <a href=\"https://dx.doi.org/10.1093/ve/vead057\" target=\"_blank\">https://dx.doi.org/10.1093/ve/vead057</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Le Lay, C.; Hamm, J.N.; Williams, T.J.; Shi, M.; Cavicchioli, R.; Holmes, E.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Le Lay, C. <i>et al.</i>","Englishabstract":"Despite their widespread distribution and remarkable  antiquity no RNA    viruses defnitively associated with the domain Archaea have  been identifed.    In contrast, 17 families of DNA viruses are known to infect  archaea. In an    attempt to uncover more of the elusive archaeal virosphere, we  investigated    the metatranscriptomes of hypersaline lakes that are a rich source  of    archaea. We sequenced RNA extracted from water flter samples of Lake Tyrrell    (Victoria, Australia) and cultures seeded from four lakes in Antarctica. To    identify highly divergent viruses in these data, we employed a variety of    search tools, including Hidden Markov models (HMMs) and positionspecifc    scoring  matrices (PSSMs). From this, we identifed 12 highly divergent, RNA    virus-like  candidate sequences from the virus phyla Artverviricota,    Duplornaviricota,  Kitrinoviricota, Negarnaviricota, and Pisuviricota,    including those with  similarity to the RNA-dependent RNA polymerase (RdRp).    An additional analysis  with an artifcial intelligence (AI)-based approach    that utilises both sequence  and structural information identifed seven    putative and highly divergent RdRp  sequences of uncertain phylogenetic    position. A sequence matching the  Pisuviricota from Deep Lake in Antarctica    had the strongest RNA virus signal.  Analyses of the dinucleotide    representation of the virus-like candidates in  comparison to that of    potential host species were in some cases compatible with  an association to    archaeal or bacterial hosts. Notably, however, the use of  archaeal CRISPR    spacers as a BLAST database failed to detect any RNA viruses.  We also    described DNA viruses from the families Pleolipoviridae,    Sphaerolipoviridae, Halspiviridae, and the class Caudoviricetes. Although we    were unable to provide defnitive evidence the existence of an RNA virus of    archaea in these hypersaline lakes, this study lays the foundations for    further  investigations of highly divergent RNA viruses in natural    environments","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Viral community composition of hypersaline lakes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"archaea; hypersaline; RNA virus; bacteriophage; detection; RNA-dependent; RNA polymerase","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2023-11-15","DateCreate":"2023-11-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001061349700001","VABBcode":null,"OpenAcc":1,"DOI":"10.1093/ve/vead057"},"refs":null,"anarec":{"AnaID":368700,"PubliDate":2023,"Pagination":null,"XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":262796,"SerRR":"Advances in Virus Research. 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