{"refrec":{"BRefID":369339,"RR":"<b>Smith, R.L.; England, A.; Millis, J.; Hirn, C.; Collins, S.D.; Connell, L.B.</b> (2023). A microfabricated, flow-driven grinding mill for mechanical cell lysing. <i>Anal. Chem. 95(48)</i>: 17494-17501. <a href=\"https://dx.doi.org/10.1021/acs.analchem.3c02344\" target=\"_blank\">https://dx.doi.org/10.1021/acs.analchem.3c02344</a>","BEntID":367069,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Anal. Chem. 95(48)</i>: 17494-17501. <a href=\"https://dx.doi.org/10.1021/acs.analchem.3c02344\" target=\"_blank\">https://dx.doi.org/10.1021/acs.analchem.3c02344</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Smith, R.L.; England, A.; Millis, J.; Hirn, C.; Collins, S.D.; Connell, L.B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Smith, R.L. <i>et al.</i>","Englishabstract":"This paper presents the design, microfabrication, and demonstration of a novel microfluidic grinding mill for the lysis of the dinoflagellate, <i>Alexandrium</i>, a neurotoxin-producing genus of algae that is responsible for red tide and paralytic shellfish poisoning. The mill consists of a high-speed, hydrodynamically driven microrotor coupled to a micro grinding mill that lyses robust algal cells by mechanical abrasion with single-pass efficiencies as high as 97%. These efficiencies are comparable to, or better than, current mechanical and chemical lysing methods without adding complications associated with harsh chemical additives that can interfere with subsequent downstream bioanalysis. 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