Document of bibliographic reference 37831

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Initiation and development of cichlid and zebrafish first-generation teeth: an in vitro study

Abstract

We have used a recently developed organ culture method based on a serum-free culture medium, appropriate for teleost skeletal and dental tissues (KOUMANS & SIRE, 1996), to study the initiation and development of first-generation teeth in early post-embryonic stages of two teleostean fishes, the cichlid Hemichromis bimaculatus (Cichlidae) and the zebrafish Danio rerio (Cyprinidae). Entire heads, explanted for 3 to 7 days reveal (1) the in vitro formation of tooth germs from a microscopically undifferentiated epithelium in the cichlid, but not in the zebrafish, (2) ongoing morphogenesis and cytodifferentiation of initiated tooth germs including the deposition of dentine, and (3) attachment of the tooth through attachment bone deposited in vitro. The pattern observed in early postembryonic stages of the cichlid can be mimicked in organ cultures of the head, but not of isolated jaws. The results point to the possibility of the involvement of neurotrophic factors in tooth initiation in these teleost models.

Bibliographic citation

Huysseune, A.; Sire, J.-Y.; Van der Heyden, C. (1998). Initiation and development of cichlid and zebrafish first-generation teeth: an in vitro study. Biol. Jb. Dodonaea 65: 156

Topic

Fresh water