{"refrec":{"BRefID":380568,"RR":"<b>Lund, P.K.</b> (2023). Host-microbe interactions in non-native estuarine anemones: biogeography and temperature. Cal Poly Humboldt theses and projects. 628.  MSc Thesis. Faculty of California State Polytechnic University: Humboldt. 143 pp.","BEntID":378309,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". Cal Poly Humboldt theses and projects. 628.  MSc Thesis. Faculty of California State Polytechnic University: Humboldt.  143 pp.","DocTypID":5,"DocType":"Book/Monograph","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lund, P.K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lund, P.K.","Englishabstract":"Non-native species are increasing in prevalence around the world, resulting in negative economic and ecological impacts. However, the broad distributions of non-native species also offer a system for investigating the response of host-associated microbial communities to environmental factors across a range of ecological scales. At the broadest scale, I investigated the geography of microbial communities in the non-native estuarine anemone <i>Diadumene lineata</i> on the west coast of the United States of America. Across latitudes, microbial community composition was very similar and displayed a high percentage of <i>Klebsiella spp. </i>at all sites. However, the communities in California tended to exhibit higher richness, Shannon-Wiener diversity, and beta-dispersion than the communities in Oregon and Washington, driven by an abundance of <i>Desulfobacterota</i>. In a stress experiment, where three anemone species (<i>Diadumene lineata</i>, <i>Diadumene leucolena</i>, and <i>Metridium senile</i>) were subjected to a gradient from 0-40°C to evaluate each species’ capacity for buffering their microbial community against thermal stress, I found species-specific patterns across temperatures, with only <i>M. senile </i>exhibiting evidence of buffering at moderate temperatures. In contrast, <i>D. lineata</i> and <i>D. leucolena </i>did not appear to be buffering their microbial communities, with <i>D. lineata</i> displaying unique community compositions across temperatures, while the communities on <i>D. leucolena</i> generally exhibited high beta-dispersion. Finally, I isolated anemone-associated bacteria on a novel medium made of anemone tissue and measured their growth from 30-40°C, identifying candidates for beneficial host-microbe interactions in warm environments. The anemone-based medium overwhelmingly selected for the genera <i>Pseudoalteromonas</i> and <i>Peribacillis,</i> regardless of anemone host species. <i>Peribacillus spp.</i> were particularly thermal tolerant, growing similarly from 30-40°C, while <i>Pseudoalteromonas spp.</i> grew well from 30-35°C. The remaining tested genera preferred 30°C, however one of the <i>Litoreibacter sp.</i> produced a putative melanin that may protect cells against thermal stress. This is the first study exploring microbial communities in the non-native estuarine anemone <i>D. lineata</i> and lays the foundation for an expanded global assessment of latitudinal gradients, investigating how additional abiotic factors like genotype and pH drive microbial community composition, and directly testing beneficial host-microbe interactions with isolated bacteria.","AbstractOtherLang":null,"BibLvlCode":"M","StandardTitle":"Host-microbe interactions in non-native estuarine anemones: biogeography and temperature","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":null,"MonPub":2023,"DateUpdate":"2024-01-08","DateCreate":"2024-01-08","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":null,"monrec":{"MonID":380568,"ISBN":null,"PubliDate":2023,"IssueDate":null,"Volume":null,"Issue":null,"Pagination":"143","Place":"Humboldt","Edition":"Cal Poly Humboldt theses and projects. 628. 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