{"refrec":{"BRefID":381110,"RR":"<b>Abedi, H.; Shahpiri, A.</b> (2024). Functional characterization of a manganese superoxide dismutase from <i>Avicennia marina</i>: insights into its role in salt, hydrogen peroxide, and heavy metal tolerance. <i>NPG Scientific Reports 14(1)</i>: 406. <a href=\"https://dx.doi.org/10.1038/s41598-023-50851-5\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-023-50851-5</a>","BEntID":378851,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 14(1)</i>: 406. <a href=\"https://dx.doi.org/10.1038/s41598-023-50851-5\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-023-50851-5</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Abedi, H.; Shahpiri, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Abedi, H.; Shahpiri, A.","Englishabstract":"<i>Avicennia marina</i> is a salt-tolerance plant with high antioxidant and antibacterial potential. In the present work, a gene encoding MnSOD from <i>Avicennia marina</i> (AmSOD2) was cloned in the expression vectors pET28a. The resulting constructs were transformed into <i>Escherichia coli</i> strains Rosetta (DE3). Following the induction with Isopropyl β-D-1-thiogalactopyranoside, the protein His-AmSOD2 was expressed but dominantly found in the insoluble fraction of strain R-AmSOD2. Due to detection of mitochondrial transit peptide in the amino acid sequence of AmSOD2, the transit peptide was removed and AmSOD2 without transit peptide (tAmSOD2) was expressed in <i>E. coli</i> and dominantly found in the soluble fraction. The enzyme His-tAmSOD2 exhibited a molecular mass of 116 kDa in native condition. Nevertheless, in reducing conditions the molecular mass is 28 kDa indicating the enzyme His-tAmSOD2 is a tetramer protein. As shown by ICP analysis there is one mole Mn<sup>2+</sup> in each monomer. The Pure His-tAmSOD2 was highly active in vitro<i>,</i> however the activity was almost three-fold lower than His-AmSOD1. Whereas the high stability of the recombinant His-AmSOD1was previously shown after incubation in a broad range pH and high temperature, His-tAmSOD2 was stable up to 50&nbsp;°C and pH 6 for 1 h. The gene expression analysis showed that the gene encoding AmSOD2 is expressed in root, shoot and leaves of <i>A. marina</i>. In addition, the results show that the expression in the leaves was enhanced after treatment of plant with NaCl, H<sub>2</sub>O<sub>2</sub>, Cd<sup>2+</sup> and Ni<sup>2+</sup> indicating the important role of MnSOD in the resistant mechanism of mangroves.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Functional characterization of a manganese superoxide dismutase from <i>Avicennia marina</i>: insights into its role in salt, hydrogen peroxide, and heavy metal tolerance","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-06 01:35:14.353791","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-01-22","DateCreate":"2024-01-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-023-50851-5"},"refs":null,"anarec":{"AnaID":381110,"PubliDate":2024,"Pagination":"406","XtraPublOfAnaID":null,"ISBN":null,"Volume":"14","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":406,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Abedi","Firstname":"Hamid","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":378851,"AutID":550508,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Shahpiri","Firstname":"Azar","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":378851,"AutID":550509,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":395446,"LibID":36,"BRefID":381110,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"395446","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Avicennia marina"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":378851,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":378851,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-023-50851-5","externalID":"10.1038/s41598-023-50851-5","URLTypeCode":"DOI","URLID":122038,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Avicennia marina [grey mangrove]","AphiaID":235040,"TaxtID":25202}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Avicennia marina","DutchTerm":null,"AphiaID":235040,"TaxtID":25202}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2024-01-22 08:20:48.030000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2024-01-22 08:20:48.030000","timezone_type":3,"timezone":"Europe/Brussels"}}}