{"refrec":{"BRefID":381701,"RR":"<b>Kyslík, J.; Born-Torrijos, A.; Holzer, A.S.; Kosakyan, A.</b> (2024). RNAi-directed knockdown in the cnidarian fish blood parasite <i>Sphaerospora molnari</i>. <i>NPG Scientific Reports 14(1)</i>: 3545. <a href=\"https://dx.doi.org/10.1038/s41598-024-54171-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-024-54171-0</a>","BEntID":379442,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 14(1)</i>: 3545. <a href=\"https://dx.doi.org/10.1038/s41598-024-54171-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-024-54171-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kyslík, J.; Born-Torrijos, A.; Holzer, A.S.; Kosakyan, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kyslík, J. <i>et al.</i>","Englishabstract":"RNA interference (RNAi) is an effective approach to suppress gene expression and monitor gene regulation. Despite its wide application, its use is limited in certain taxonomic groups, including cnidarians. Myxozoans are a unique group of cnidarian parasites that diverged from their free-living ancestors about 600 million years ago, with several species causing acute disease in farmed and wild fish populations. In this pioneering study we successfully applied RNAi in blood stages of the myxozoan <i>Sphaerospora molnari</i>, combining a dsRNA soaking approach, real-time PCR, confocal microscopy, and Western blotting. For proof of concept, we knocked down two unusual actins, one of which is known to play a critical role in <i>S</i>. <i>molnari</i> cell motility. We observed intracellular uptake of dsRNA after 30&nbsp;min and accumulation in all cells of the typical myxozoan cell-in-cell structure. We successfully knocked down actin in <i>S</i>. <i>molnari </i>in vitro<i>,</i> with transient inhibition for 48&nbsp;h. We observed the disruption of the cytoskeletal network within the primary cell and loss of the characteristic rotational cell motility. This RNAi workflow could significantly advance functional research within the Myxozoa, offering new prospects for investigating therapeutic targets and facilitating drug discovery against economically important fish parasites.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"RNAi-directed knockdown in the cnidarian fish blood parasite <i>Sphaerospora molnari</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:32:06.248096","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Sphaerospora molnari","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-02-12","DateCreate":"2024-02-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-024-54171-0"},"refs":null,"anarec":{"AnaID":381701,"PubliDate":2024,"Pagination":"3545","XtraPublOfAnaID":null,"ISBN":null,"Volume":"14","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":3545,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Kyslík","Firstname":"Jirí","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":379442,"AutID":552848,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Born-Torrijos","Firstname":"Ana","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":379442,"AutID":470048,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Holzer","Firstname":"Astrid","Initials":"A.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":379442,"AutID":552850,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kosakyan","Firstname":"Anush","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":379442,"AutID":385036,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":396002,"LibID":36,"BRefID":381701,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"396002","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":379442,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":379442,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-024-54171-0","externalID":"10.1038/s41598-024-54171-0","URLTypeCode":"DOI","URLID":127872,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2024-02-12 13:27:01.727000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2024-02-12 13:27:01.727000","timezone_type":3,"timezone":"Europe/Brussels"}}}