Seafood is a major food source worldwide, but it is prone to fraudulent activities such as species substitution. DNA barcoding is currently the most used tool to identify processed seafood, but remains expensive, time-consuming, and requires heavy and expensive lab equipment and expert knowledge. Here we compared the Nucleospin® Food kit with a dipstick-based, a paramagnetic bead-based, and an alkaline-based DNA extraction method on tissue of common sole (Solea solea). The alkaline-based method was the most reliable and cheapest, with the lowest hands-on time. A S. solea-specific loop-mediated isothermal amplification (LAMP) assay was designed using the cytochrome b (cytb) gene with a limit of detection (LOD) of 0.1 ng. The alkaline-based DNA extraction and LAMP was validated using ten sole fillets under different preservation conditions (fresh, frozen, and ethanol stored), and ten previously identified sole dishes. A combination of the alkaline-based DNA extraction and the LAMP assay detects common sole in seafood products within an hour in the field, and at a cost of less than half a euro. The method developed in this study is applicable in large-scale audits of sole products. Similar methods may emerge for other seafood species allowing seafood fraud studies in labs short on resources.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Rapid and low-cost identification of common sole (Solea solea) in the field using a fast DNA isolation protocol and loop-mediated isothermal amplification (LAMP)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:56.995835","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Authentication; cytochrome b; DNA isolation; Isothermal amplification; Point-of-care; Seafood fraud; Substitution","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2024-02-19","DateCreate":"2024-02-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000961155300001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jfca.2023.105166"},"refs":null,"anarec":{"AnaID":382946,"PubliDate":2023,"Pagination":"105166","XtraPublOfAnaID":null,"ISBN":null,"Volume":"118","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":116155,"SerRR":"Journal of Food Composition and Analysis. Elsevier: San Diego. ISSN 0889-1575; e-ISSN 1096-0481","StandardTitleSer":"Journal of Food Composition and Analysis","ISSN":"0889-1575","AbbrevSer":"J. food compos. anal. (Print)","StandardTitleMon":null,"StartPage":9,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Deconinck","Firstname":"Dumas","Initials":"D.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food, ILVO, Aquatic Environment and Quality","Discriminator":null,"CorporateFlag":0,"BEntID":380688,"AutID":421320,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw- , visserij en voedingsonderzoek; Kenniseenheid: Dier; Aquatisch Milieu en Kwaliteit","ORCID":"0000-0002-2854-7708","PersID":36999,"InsID":12745},{"AutName":"Robbens","Firstname":"Johan","Initials":"J.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food, ILVO, Aquatic Environment and Quality","Discriminator":null,"CorporateFlag":0,"BEntID":380688,"AutID":421327,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw- , visserij en voedingsonderzoek; Kenniseenheid: Dier; Aquatisch Milieu en Kwaliteit","ORCID":null,"PersID":12105,"InsID":12745},{"AutName":"Volckaert","Firstname":"Filip","Initials":"F.A.M.","Affiliation":"Laboratory of Biodiversity and Evolutionary Genomics, KU Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":380688,"AutID":157751,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"LBEG","InsFSN":"KU Leuven; Departement Biologie; Ecology, Evolution and Biodiversity Conservation Section; Laboratorium voor Biodiversiteit en Evolutionaire Genomica","ORCID":"0000-0003-4342-4295","PersID":13,"InsID":12175},{"AutName":"Derycke","Firstname":"Sofie","Initials":"S.","Affiliation":"Flanders Research Institute for Agriculture, Fisheries and Food, ILVO, Aquatic Environment and Quality","Discriminator":null,"CorporateFlag":0,"BEntID":380688,"AutID":421328,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw- , visserij en voedingsonderzoek; Kenniseenheid: Dier; Aquatisch Milieu en Kwaliteit","ORCID":"0000-0003-3763-6187","PersID":5433,"InsID":12745}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":396264,"LibID":36,"BRefID":382946,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"396264","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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