The marine isolate Alteromonas macleodii Mo 169 secretes a exopolysaccharide (EPS) mainly composed of glucuronic acid and containing sulphate, pyruvate, and lactate as acyl substituents. The high density of negatively charged functional groups of the EPS renders it the ability to function as a stabilizing and reducing agent for the synthesis of metallic nanoparticles (NP). In this study, silver and gold NP (AgNP and AuNP, respectively) were successfully synthesized and stabilized by A. macleodii Mo 169 EPS. The obtained AuNP and AgNP presenting a spherical shape with an average particle size of 16 and 15 nm, respectively, displaying a polysaccharide layer that apparently contributed to reduce their aggregation. The cytotoxicity of the EPS and the bio-nanocomposites was evaluated on human keratinocyte (HaCaT) and fibroblast (CCD-1079Sk) cell lines. None of the samples demonstrated cytotoxicity for concentrations up to 1000 mg L−1. In vitro wound healing experiments demonstrated that both bio-nanocomposites promoted cell migration for concentrations above 100 mg L−1, thus contributing for a faster wound recovery. These findings demonstrate for the first time the potential of A. macleodii Mo169 EPS to synthesize and stabilize nanoparticles that possess promising physical-chemical and biological characteristics that render them valuable for the development of novel biomaterials for several applications, including wound care.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Bioactive exopolysaccharide-composites based on gold and silver nanoparticles tailored for wound healing","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Alteromonas macleodii Mo 169; Exopolysaccharide (EPS); Silver nanoparticles (AgNP); Gold nanoparticles (AuNP); Bioactive material","OtherDescriptors":null,"Notes":null,"AnaPub":2023,"MonPub":null,"DateUpdate":"2024-02-19","DateCreate":"2024-02-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000991159500001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.mtcomm.2023.105351"},"refs":null,"anarec":{"AnaID":382952,"PubliDate":2023,"Pagination":"105351","XtraPublOfAnaID":null,"ISBN":null,"Volume":"34","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":274395,"SerRR":"Materials Today Communications. Elsevier: Amsterdam. e-ISSN 2352-4928","StandardTitleSer":"Materials Today Communications","ISSN":null,"AbbrevSer":null,"StandardTitleMon":null,"StartPage":10,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Concordio-Reis","Firstname":"Patricia","Initials":"P.","Affiliation":"NOVA Univ Lisbon, Inst Hlth & Bioecon, Sch Sci & Technol, Associate Lab i4HB, P-2829516 Caparica, Portugal.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556088,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ramos","Firstname":"Kleyde","Initials":"K.","Affiliation":"NOVA Univ Lisbon, Inst Hlth & Bioecon, Sch Sci & Technol, Associate Lab i4HB, P-2829516 Caparica, Portugal.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556090,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Macedo","Firstname":"Ana Catarina","Initials":"A.C.","Affiliation":"Inst Biol Expt & Tecnol, iBET, Apartado 12, P-2781901 Oeiras, Portugal.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556092,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Serra","Firstname":"Ana Teresa","Initials":"A.T.","Affiliation":"NOVA Univ Lisbon, Inst Hlth & Bioecon, Sch Sci & Technol, Associate Lab i4HB, P-2829516 Caparica, Portugal.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556094,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Moppert","Firstname":"Xavier","Initials":"X.","Affiliation":"Pacific Biotech, BP 140 289, F-98701 Arue, Tahiti, France.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556095,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Guezennec","Firstname":"Jean","Initials":"J.","Affiliation":"AiMB Adv Marine Biotechnol, 17 Rue Ouessant, F-29280 Plouzane, France.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556096,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sevrin","Firstname":"Chantal","Initials":"C.","Affiliation":"Interfaculty Research Centre of Biomaterials (CEIB), University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556097,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":44847,"InsID":null},{"AutName":"Grandfils","Firstname":"Christian","Initials":"C.","Affiliation":"Interfaculty Research Centre of Biomaterials (CEIB), University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556098,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":39163,"InsID":null},{"AutName":"Reis","Firstname":"Maria","Initials":"M.A.M.","Affiliation":"NOVA Univ Lisbon, Inst Hlth & Bioecon, Sch Sci & Technol, Associate Lab i4HB, P-2829516 Caparica, Portugal.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556099,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Freitas","Firstname":"Filomena","Initials":"F.","Affiliation":"NOVA Univ Lisbon, Inst Hlth & Bioecon, Sch Sci & Technol, Associate Lab i4HB, P-2829516 Caparica, Portugal.","Discriminator":null,"CorporateFlag":0,"BEntID":380694,"AutID":556101,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":396269,"LibID":36,"BRefID":382952,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"396269","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site
en mag op geen enkele manier verder worden verspreid.