{"refrec":{"BRefID":383434,"RR":"<b>Combosch, D.J.; Burdick, D.; Primov, K.; Rios, D.; Rios, K.; Fernandez, J.</b> (2024). Barcoding and mitochondrial phylogenetics of <i>Porites </i>corals. <i>PLoS One 19(2)</i>: e0290505. <a href=\"https://dx.doi.org/10.1371/journal.pone.0290505\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0290505</a>","BEntID":381180,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 19(2)</i>: e0290505. <a href=\"https://dx.doi.org/10.1371/journal.pone.0290505\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0290505</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Combosch, D.J.; Burdick, D.; Primov, K.; Rios, D.; Rios, K.; Fernandez, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Combosch, D.J. <i>et al.</i>","Englishabstract":"<p style=\"margin-left:0px;\">Coral reefs are the most diverse ecosystem on the planet based on the abundance and diversity of phyla and higher taxa. However, it is still difficult to assess the diversity of lower taxa, especially at the species level. One tool for improving the identification of lower taxa are genetic markers that can distinguish cryptic species and assess species boundaries. Here, we present one such approach for an important and challenging group of reef-building corals. <i>Porites</i> corals are the main reef-builders of many coral reefs in the Indo-Pacific, owing to the massive growth forms of some species. The current number of valid <i>Porites</i> species is controversial, inflated with many synonymies, and often based on gross colony morphology although several morphospecies believed to be widespread and common can only be distinguished based on detailed microstructure analyses by taxonomic experts. Here, we test the suitability of multiple regions of mtDNA as genetic barcodes to identify suitable markers for species differentiation and unambiguous identification. Resulting sequencing data was further used for the first phylogenetic analysis of Guam’s <i>Porites</i> species. We tested eight different mitochondrial markers and analyzed four in detail for 135 <i>Porites</i> specimens: mtDNA markers were amplified for 67 <i>Porites</i> specimens from Guam, representing 12 nominal <i>Porites</i> species, and combined with 69 mitochondrial genomes, mostly from Hawaii. The combination of all 4 markers distinguished 10 common and 7 uncommon Central-West Pacific <i>Porites</i> species. Most clades separate species along taxonomic boundaries, which is uncommon for <i>Porites</i> corals and testifies to the suitability of our multi-marker approach, and a combination of the two most promising barcodes distinguished 8/10 common species. These barcodes are thus suitable to distinguish virtually cryptic species in one of the most important and challenging coral genera. They offer a cheap, fast and reliable way to identify <i>Porites</i> species for species-level research, monitoring and conservation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Barcoding and mitochondrial phylogenetics of <i>Porites </i>corals","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-02-26","DateCreate":"2024-02-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0290505"},"refs":null,"anarec":{"AnaID":383434,"PubliDate":2024,"Pagination":"e0290505","XtraPublOfAnaID":null,"ISBN":null,"Volume":"19","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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