{"refrec":{"BRefID":383568,"RR":"<b>Lizano, A.M.D.; Kim, K.M.; Juinio-Meñez, M.A.; Ravago-Gotanco, R.</b> (2024). Pseudocryptic diversity and species boundaries in the sea cucumber <i>Stichopus </i>cf. <i>horrens </i>(Echinodermata: Stichopodidae) revealed by mitochondrial and microsatellite markers. <i>NPG Scientific Reports 14(1)</i>: 4886. <a href=\"https://dx.doi.org/10.1038/s41598-024-54987-w\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-024-54987-w</a>","BEntID":381314,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 14(1)</i>: 4886. <a href=\"https://dx.doi.org/10.1038/s41598-024-54987-w\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-024-54987-w</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lizano, A.M.D.; Kim, K.M.; Juinio-Meñez, M.A.; Ravago-Gotanco, R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lizano, A.M.D. <i>et al.</i>","Englishabstract":"Morphologically cryptic and pseudo-cryptic species pose a challenge to taxonomic identification and assessments of species diversity and distributions. Such is the case for the sea cucumber <i>Stichopus horrens</i>, commonly confused with <i>Stichopus monotuberculatus</i>. Here, we used mitochondrial cytochrome oxidase subunit I (COI) and microsatellite markers to examine genetic diversity in <i>Stichopus</i> cf. <i>horrens</i> throughout the Philippine archipelago, to aid species identification and clarify species boundaries. Phylogenetic analysis reveals two recently diverged COI lineages (Clade A and Clade B; <i>c</i>. 1.35–2.54 Mya) corresponding to sequence records for specimens identified as <i>S. monotuberculatus</i> and <i>S. horrens,</i> respectively. Microsatellite markers reveal two significantly differentiated genotype clusters broadly concordant with COI lineages (Cluster 1, Cluster 2). A small proportion of individuals were identified as later-generation hybrids indicating limited contemporary gene flow between genotype clusters, thus confirming species boundaries. Morphological differences in papillae distribution and form are observed for the two species, however tack-like spicules from the dorsal papillae are not a reliable diagnostic character. An additional putative cryptic species was detected within Clade B-Cluster 2 specimens warranting further examination. We propose that these lineages revealed by COI and genotype data be referred to as <i>Stichopus</i> cf. <i>horrens</i> species complex.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Pseudocryptic diversity and species boundaries in the sea cucumber <i>Stichopus </i>cf. <i>horrens </i>(Echinodermata: Stichopodidae) revealed by mitochondrial and microsatellite markers","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:32:17.115624","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-03-04","DateCreate":"2024-03-04","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-024-54987-w"},"refs":null,"anarec":{"AnaID":383568,"PubliDate":2024,"Pagination":"4886","XtraPublOfAnaID":null,"ISBN":null,"Volume":"14","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  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