{"refrec":{"BRefID":391324,"RR":"<b>Vidal, L.M.R.; Venas, T.M.; Goncalves, A.R.P.; Mattsson, H.K.; Silva, R.V.P.; Nobrega, M.S.; Azevedo, G.P.R.; Garcia, G.D.; Tschoeke, D.A.; Vieira, V.V.; Thompson, F.L.; Thompson, C.C.</b> (2020). Rapid screening of marine bacterial symbionts using MALDI-TOF MS. <i>Arch. Microbiol. 202(8)</i>: 2329-2336. <a href=\"https://dx.doi.org/10.1007/s00203-020-01917-9\" target=\"_blank\">https://dx.doi.org/10.1007/s00203-020-01917-9</a>","BEntID":389071,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Arch. Microbiol. 202(8)</i>: 2329-2336. <a href=\"https://dx.doi.org/10.1007/s00203-020-01917-9\" target=\"_blank\">https://dx.doi.org/10.1007/s00203-020-01917-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vidal, L.M.R.; Venas, T.M.; Goncalves, A.R.P.; Mattsson, H.K.; Silva, R.V.P.; Nobrega, M.S.; Azevedo, G.P.R.; Garcia, G.D.; Tschoeke, D.A.; Vieira, V.V.; Thompson, F.L.; Thompson, C.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vidal, L.M.R. <i>et al.</i>","Englishabstract":"Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including <i>Vibrio, Bacillus, Pseudovibrio, Alteromonas</i> and <i>Ruegeria</i>. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% <i>pyrH</i> gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for <i>Vibrio</i> species groups’ identification.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Rapid screening of marine bacterial symbionts using MALDI-TOF MS","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:32:43.601689","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"MALDI-TOF; Screening tool; Marine bacterial symbionts; Vibrio; pyrHgene","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2024-03-26","DateCreate":"2024-03-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000539891800002","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00203-020-01917-9"},"refs":null,"anarec":{"AnaID":391324,"PubliDate":2020,"Pagination":"2329-2336","XtraPublOfAnaID":null,"ISBN":null,"Volume":"202","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":133426,"SerRR":"Archives of Microbiology. 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