In this study, transmission electron microscopy (TEM) and cryo-scanning electron microscopy (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum and Nannochloropsis oculata cells were harvested in both the mid-exponential and early stationary growth phase. Two different cryo-SEM cutting methods were compared: cryo-planing and freeze-fracturing. The results showed that, despite the longer preparation time, TEM visualisation preceded by cryo-immobilisation allows a clear detection of lipid bodies and is preferable to cryo-SEM. Using freeze-fracturing, lipid bodies were rarely detected. This was only feasible if crystalline layers in the internal structure, most likely related to sterol esters or di-saturated triacylglycerols, were revealed. Furthermore, lipid bodies could not be detected using cryo-planing. Cryo-SEM is also not the preferred technique to recognise other organelles besides lipid bodies, yet it did reveal chloroplasts in both species and filament-containing organelles in cryo-planed Nannochloropsis oculata samples.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Visualisation of microalgal lipid bodies through electron microscopy","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-03 01:33:09.690581","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cryo-planing; electron microscopy; freeze-fracturing; lipid bodies; Nannochloropsis oculata; Phaeodactylum tricornutum","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-04-16","DateCreate":"2024-03-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001137446600001","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jmi.13259"},"refs":null,"anarec":{"AnaID":391395,"PubliDate":2024,"Pagination":"118-131","XtraPublOfAnaID":null,"ISBN":null,"Volume":"293","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45663,"SerRR":"Journal of Microscopy. Blackwell: Oxford. ISSN 0022-2720; e-ISSN 1365-2818","StandardTitleSer":"Journal of Microscopy","ISSN":"0022-2720","AbbrevSer":"J. Microsc.","StandardTitleMon":null,"StartPage":118,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Verwee","Firstname":"Ellen","Initials":"E.","Affiliation":"Department of Food Technology, Food Structure & Function research group, Safety and Health, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":389142,"AutID":562181,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0009-0007-7878-8650","PersID":45165,"InsID":null},{"AutName":"van de Walle","Firstname":"Davy","Initials":"D.","Affiliation":"Department of Food Technology, Food Structure & Function research group, Safety and Health, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":389142,"AutID":562184,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0003-1018-1696","PersID":45166,"InsID":null},{"AutName":"De Bruyne","Firstname":"Michiel","Initials":"M.","Affiliation":"VIB BioImaging Core VIB","Discriminator":null,"CorporateFlag":0,"BEntID":389142,"AutID":562185,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":36140,"InsID":null},{"AutName":"Mienis","Firstname":"Esther","Initials":"E.","Affiliation":"Research Unit Food & Lipids, KU Leuven Kulak","Discriminator":null,"CorporateFlag":0,"BEntID":389142,"AutID":562188,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"KU Leuven; 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