{"refrec":{"BRefID":391565,"RR":"<b>Mitchell, M.L.; Hossain, M.A.; Lin, F.; Pinheiro-Junior, E.L.; Peigneur, S.; Wai, D.C.C.; Delaine, C.; Blyth, A.J.; Forbes, B.E.; Tytgat, J.; Wade, J.D.; Norton, R.S.</b> (2021). Identification, synthesis, conformation and activity of an insulin-like peptide from a sea anemone. <i>Biomolecules 11(12)</i>: 1785. <a href=\"https://dx.doi.org/10.3390/biom11121785\" target=\"_blank\">https://dx.doi.org/10.3390/biom11121785</a>","BEntID":389312,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biomolecules 11(12)</i>: 1785. <a href=\"https://dx.doi.org/10.3390/biom11121785\" target=\"_blank\">https://dx.doi.org/10.3390/biom11121785</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Mitchell, M.L.; Hossain, M.A.; Lin, F.; Pinheiro-Junior, E.L.; Peigneur, S.; Wai, D.C.C.; Delaine, C.; Blyth, A.J.; Forbes, B.E.; Tytgat, J.; Wade, J.D.; Norton, R.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Mitchell, M.L. <i>et al.</i>","Englishabstract":"<p style=\"margin-left:0px;\">The role of insulin and insulin-like peptides (ILPs) in vertebrate animals is well studied. Numerous ILPs are also found in invertebrates, although there is uncertainty as to the function and role of many of these peptides. We have identified transcripts with similarity to the insulin family in the tentacle transcriptomes of the sea anemone <i>Oulactis</i> sp. (Actiniaria: Actiniidae). The translated transcripts showed that these insulin-like peptides have highly conserved A- and B-chains among individuals of this species, as well as other Anthozoa. An <i>Oulactis</i> sp. ILP sequence (IlO1_i1) was synthesized using Fmoc solid-phase peptide synthesis of the individual chains, followed by regioselective disulfide bond formation of the intra-A and two interchain disulfide bonds. Bioactivity studies of IlO1_i1 were conducted on human insulin and insulin-like growth factor receptors, and on voltage-gated potassium, sodium, and calcium channels. IlO1_i1 did not bind to the insulin or insulin-like growth factor receptors, but showed weak activity against K<sub>V</sub>1.2, 1.3, 3.1, and 11.1 (hERG) channels, as well as Na<sub>V</sub>1.4 channels. Further functional studies are required to determine the role of this peptide in the sea anemone.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification, synthesis, conformation and activity of an insulin-like peptide from a sea anemone","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Oulactis; cnidaria; peptide synthesis; ion channel; invertebrates; insulin","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2024-03-26","DateCreate":"2024-03-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000736199100001","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/biom11121785"},"refs":null,"anarec":{"AnaID":391565,"PubliDate":2021,"Pagination":"1785","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":264565,"SerRR":"Biomolecules. 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