{"refrec":{"BRefID":391773,"RR":"<b>Lagaisse, R.<\/b> (2024). LifeWatch Belgium: FlowCam sampling and lab protocol for imaging microphytoplankton in the Belgian part of the North Sea. Flanders Marine Institute: Oostende. 10 pp. <a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.6qpvr8e6zlmk\/v1\" target=\"_blank\">https:\/\/dx.doi.org\/10.17504\/protocols.io.6qpvr8e6zlmk\/v1<\/a>","BEntID":389520,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". Flanders Marine Institute: Oostende.  10 pp. <a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.6qpvr8e6zlmk\/v1\" target=\"_blank\">https:\/\/dx.doi.org\/10.17504\/protocols.io.6qpvr8e6zlmk\/v1<\/a>","DocTypID":5,"DocType":"Book\/Monograph","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lagaisse, R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lagaisse, R.","Englishabstract":"In the framework of the Belgian Lifewatch RI, a number of fi xed stations in the Belgian Part of the North Sea (BPNS) are visited during mulitdisciplinary campaigns. A grid of nine stations covers the coastal zone and are sampled monthly. Eight additional stations, located more offshore, are sampled on a seasonal basis. For the phytoplankton monitoring, samples are taken using a 55\u03bcm mesh size Apstein net, fi xed in Lugol's iodine solution and preserved in dark and refrigerated conditions. In the lab, the samples are processed using a FlowCAM VS-4 benchtop model, equipped with a Sony XCD SC90 digital gray-scale camera and VisualSpreadsheet software version 4.2.52. For the monitoring, the FlowCam device is mounted with the 4X magnifi cation and associated hardware to image a particles size range of 55-300\u03bcm. The identifi cation of the images data is done with a CNN and followed by a manual validation step to check each predicated image. Since May 2017, this dataset provides micro- and phytoplankton observations, mainly covering diatoms, dinofl agellates and cilliates, for the Belgian Part of the North Sea (BPNS). This published protocol descibes sampling and lab SOPs in detail.","AbstractOtherLang":null,"BibLvlCode":"M","StandardTitle":"LifeWatch Belgium: FlowCam sampling and lab protocol for imaging microphytoplankton in the Belgian part of the North Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-02-08 01:31:01.579764","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"FlowCam, SOP, LifeWatch","OtherDescriptors":null,"Notes":null,"AnaPub":null,"MonPub":2024,"DateUpdate":"2024-04-15","DateCreate":"2024-04-15","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.17504\/protocols.io.6qpvr8e6zlmk\/v1"},"refs":null,"anarec":null,"monrec":{"MonID":391773,"ISBN":null,"PubliDate":2024,"IssueDate":null,"Volume":null,"Issue":null,"Pagination":"10","Place":"Oostende","Edition":null,"BRefXtra":null,"BRefXtraRR":null,"SerID":null,"SerRR":null,"Ser2BRefID":null,"Ser2RR":null,"StandardTitleSer":null,"ISSN":null,"AbbrevSer":null,"Degree":null,"ThesisID":null,"InsID":null,"Acronym":null,"FullStandardName":null,"ToPubliDate":null,"SerNotes":null,"eISBN":null,"Pages":10},"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lagaisse","Firstname":"Rune","Initials":"R.","Affiliation":"Flanders Marine Institute (VLIZ)","Discriminator":null,"CorporateFlag":0,"BEntID":389520,"AutID":476645,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"VLIZ","InsFSN":"Vlaams Instituut voor de Zee","ORCID":"0000-0001-9191-9140","PersID":39431,"InsID":36}],"mapdetails":null,"datasets":[{"DasID":4688,"Acronym":null,"Citation":"Flanders Marine Institute (VLIZ), Belgium (2026): LifeWatch observatory data: phytoplankton observations by FlowCam imaging in the Belgian Part of the North Sea.","StandardTitle":"LifeWatch observatory data: phytoplankton observations by FlowCam imaging in the Belgian Part of the North Sea","RelationID":2,"DOI":"10.14284\/774"}],"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":400053,"LibID":36,"BRefID":391773,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"400053","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https:\/\/www.vliz.be\/imisdocs\/publications\/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":null,"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Phytoplankton","ThestID":6208,"Acronym":"ASFA","ThesTermPath":"Aquatic communities > Plankton > Phytoplankton"},{"ThesaurusTerm":"Marine","ThestID":75944,"Acronym":"CSA","ThesTermPath":"Marine"},{"ThesaurusTerm":"Monitoring","ThestID":5312,"Acronym":"ASFA","ThesTermPath":"Monitoring"}],"thestermsASFA":[{"ThesaurusTerm":"Marine"},{"ThesaurusTerm":"Monitoring"},{"ThesaurusTerm":"Phytoplankton"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - 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