{"refrec":{"BRefID":392755,"RR":"<b>Salazar Alekseyeva, K.; Herndl, G.; Baltar, F.</b> (2024). Influence of salinity on the extracellular enzymatic activities of marine pelagic fungi. <i>Journal of Fungi 10(2)</i>: 152. <a href=\"https://dx.doi.org/10.3390/jof10020152\" target=\"_blank\">https://dx.doi.org/10.3390/jof10020152</a>","BEntID":390519,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Journal of Fungi 10(2)</i>: 152. <a href=\"https://dx.doi.org/10.3390/jof10020152\" target=\"_blank\">https://dx.doi.org/10.3390/jof10020152</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Salazar Alekseyeva, K.; Herndl, G.; Baltar, F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Salazar Alekseyeva, K.; Herndl, G.; Baltar, F.","Englishabstract":"<span style=\"background-color:rgb(255,255,255);color:rgb(34,34,34);\">Even though fungi are ubiquitous in the biosphere, the ecological knowledge of marine fungi remains rather rudimentary. Also, little is known about their tolerance to salinity and how it influences their activities. Extracellular enzymatic activities (EEAs) are widely used to determine heterotrophic microbes’ enzymatic capabilities and substrate preferences. Five marine fungal species belonging to the most abundant pelagic phyla (Ascomycota and Basidiomycota) were grown under non-saline and saline conditions (0 g/L and 35 g/L, respectively). Due to their sensitivity and specificity, fluorogenic substrate analogues were used to determine hydrolytic activity on carbohydrates (<i>β</i>-glucosidase, <i>β</i>-xylosidase, and <i>N</i>-acetyl-<i>β</i>-D-glucosaminidase); peptides (leucine aminopeptidase and trypsin); lipids (lipase); organic phosphorus (alkaline phosphatase), and sulfur compounds (sulfatase). Afterwards, kinetic parameters such as maximum velocity (V</span><sub>max</sub><span style=\"background-color:rgb(255,255,255);color:rgb(34,34,34);\">) and half-saturation constant (K</span><sub>m</sub><span style=\"background-color:rgb(255,255,255);color:rgb(34,34,34);\">) were calculated. All fungal species investigated cleaved these substrates, but some species were more efficient than others. Moreover, most enzymatic activities were reduced in the saline medium, with some exceptions like sulfatase. In non-saline conditions, the average V</span><sub>max</sub><span style=\"background-color:rgb(255,255,255);color:rgb(34,34,34);\"> ranged between 208.5 to 0.02 μmol/g biomass/h, and in saline conditions, 88.4 to 0.02 μmol/g biomass/h. The average K</span><sub>m</sub><span style=\"background-color:rgb(255,255,255);color:rgb(34,34,34);\"> ranged between 1553.2 and 0.02 μM with no clear influence of salinity. Taken together, our results highlight a potential tolerance of marine fungi to freshwater conditions and indicate that changes in salinity (due to freshwater input or evaporation) might impact their enzymatic activities spectrum and, therefore, their contribution to the oceanic elemental cycles.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Influence of salinity on the extracellular enzymatic activities of marine pelagic fungi","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":" Ascomycota; Basidiomycota; yeast; filamentous fungi; Michaelis–Menten kinetics; salinity effect","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-06-12","DateCreate":"2024-06-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.3390/jof10020152"},"refs":null,"anarec":{"AnaID":392755,"PubliDate":2024,"Pagination":"152","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":347345,"SerRR":"Journal of Fungi. MDPI: Basel.  e-ISSN 2309-608X","StandardTitleSer":"Journal of Fungi","ISSN":null,"AbbrevSer":null,"StandardTitleMon":null,"StartPage":152,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Salazar Alekseyeva","Firstname":"Katherine","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":390519,"AutID":487486,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Herndl","Firstname":"Gerhard","Initials":"G.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":390519,"AutID":214459,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-2223-2852","PersID":8626,"InsID":13655},{"AutName":"Baltar","Firstname":"Federico","Initials":"F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":390519,"AutID":198452,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":390519,"BEntPrivate":null,"availability":[{"BInstID":406882,"LibID":2779,"BRefID":392755,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"406882","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":21,"AccessConstraint":"Attribution (CC BY)","LicURL":"https://creativecommons.org/licenses/by/4.0/"}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"MDPI","City":"Basel"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":390519,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":390519,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.3390/jof10020152","externalID":"10.3390/jof10020152","URLTypeCode":"DOI","URLID":131110,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"daan.van.loon@nioz.nl","newSesDate":{"date":"2024-06-12 09:37:13.230000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"daan.van.loon@nioz.nl","updSesDate":{"date":"2024-06-12 09:37:13.230000","timezone_type":3,"timezone":"Europe/Brussels"}}}