{"refrec":{"BRefID":393363,"RR":"<b>Abdel-Gaber, R.; Alojayri, G.; Al Quraishy, S.; Al-Shaebi, E.M.; Mohammed, O.B.</b> (2024). Morphological and molecular studies of <i>Hysterothylacium thalassini</i> third-stage larvae (Ascaridida, Raphidascarididae) in the greater lizardfish <i>Saurida tumbil</i>. <i>Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 76(4)</i>: e13206. <a href=\"https://dx.doi.org/10.1590/1678-4162-13206\" target=\"_blank\">https://dx.doi.org/10.1590/1678-4162-13206</a>","BEntID":391149,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 76(4)</i>: e13206. <a href=\"https://dx.doi.org/10.1590/1678-4162-13206\" target=\"_blank\">https://dx.doi.org/10.1590/1678-4162-13206</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Abdel-Gaber, R.; Alojayri, G.; Al Quraishy, S.; Al-Shaebi, E.M.; Mohammed, O.B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Abdel-Gaber, R. <i>et al.</i>","Englishabstract":"<i>Hysterothylacium</i> species are perhaps the most abundant and diverse group of marine ascaridoids. Identification of larval stages at specific levels is very problematic. This study describes the occurrence of <i>Hysterothylacium</i> larvae parasitizing the peritoneal cavity and mesenteries of <i>Saurida tumbil</i> purchased from fish markets in Saudi Arabia. Fish were visually inspected for nematodes using micro- and macroscopic examinations. Nematodes were assigned to genus level based on morphology and identified at specific level by sequence analyses of <i>18S rRNA</i> and <i>COI</i> genes. Morphological examination by light microscopy showed that worms identified as third-stage larvae (L3) belonged to genus <i>Hysterothylacium</i> Ward and Magath, 1917. COI sequences from host confirmed identity of host as <i>S. tumbil</i> as it resulted in identical sequences from <i>S. tumbil</i> in GenBank. Identity of L3 based on 18S rDNA sequences confirmed their identity as <i>H. thalassini</i> and showed high similarity to sequences in GenBank. A unique hitherto sequences of L3 related to COI region have been reported herein. This is the first record of <i>Hysterothylacium</i> L3-stage parasitizing lizardfish in Saudi Arabia. Therefore, this study represents the importance of a combination of morphological and molecular tools for taxonomy and systematics of ascaridoids at specific level and confirming its host identity.","AbstractOtherLang":"As espécies de Hysterothylacium são talvez o grupo mais abundante e diversificado de ascaridóides marinhos. A identificação dos estágios larvais em nível específico é muito problemática. Este estudo descreve a ocorrência de larvas de Hysterothylacium parasitando a cavidade peritoneal e os mesentérios de Saurida tumbil, comprados em mercados de peixe na Arábia Saudita. Os peixes foram inspecionados visualmente em busca de nematoides por meio de exames micro e macroscópicos. Os nematoides foram classificados em nível de gênero com base na morfologia e identificados em nível específico por meio de análises de sequência dos genes 18S rRNA e COI. O exame morfológico por microscopia de luz mostrou que os vermes identificados como larvas de terceiro estágio (L3) pertenciam ao gênero Hysterothylacium Ward e Magath, 1917. As sequências de COI do hospedeiro confirmaram a identidade do hospedeiro como S. tumbil, pois resultaram em sequências idênticas de S. tumbil no GenBank. A identidade da L3 com base nas sequências de rDNA 18S confirmou sua identidade como H. thalassini e mostrou alta similaridade com as sequências do GenBank. Uma sequência única de L3 relacionada à região COI foi relatada aqui. Esse é o primeiro registro do estágio L3 do Hysterothylacium parasitando peixes-lagarto na Arábia Saudita. Portanto, este estudo representa a importância de uma combinação de ferramentas morfológicas e moleculares para a taxonomia e a sistemática de ascaridóides em nível específico e para a confirmação da identidade de seu hospedeiro.","BibLvlCode":"AS","StandardTitle":"Morphological and molecular studies of <i>Hysterothylacium thalassini</i> third-stage larvae (Ascaridida, Raphidascarididae) in the greater lizardfish <i>Saurida tumbil</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"AuthorKeywords":"Ascaridoidea; larvae; identification; <i>Saurida</i> species; Saudi Arabia","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-07-22","DateCreate":"2024-07-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1590/1678-4162-13206"},"refs":null,"anarec":{"AnaID":393363,"PubliDate":2024,"Pagination":"e13206","XtraPublOfAnaID":null,"ISBN":null,"Volume":"76","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":263863,"SerRR":"Arquivo Brasileiro de Medicina Veterinaria e Zootecnia: Minas Gerais.  ISSN 0102-0935; e-ISSN 1678-4162","StandardTitleSer":"Arquivo Brasileiro de Medicina Veterinaria e Zootecnia","ISSN":"0102-0935","AbbrevSer":null,"StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Abdel-Gaber","Firstname":null,"Initials":"R.","Affiliation":"King Saud University,  Saudi Arabia","Discriminator":null,"CorporateFlag":0,"BEntID":391149,"AutID":569671,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Alojayri","Firstname":null,"Initials":"G.","Affiliation":"King Saud University,  Saudi Arabia","Discriminator":null,"CorporateFlag":0,"BEntID":391149,"AutID":569672,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Al Quraishy","Firstname":null,"Initials":"S.","Affiliation":"King Saud University,  Saudi Arabia","Discriminator":null,"CorporateFlag":0,"BEntID":391149,"AutID":569673,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Al-Shaebi","Firstname":null,"Initials":"E.M.","Affiliation":"King Saud University,  Saudi Arabia","Discriminator":null,"CorporateFlag":0,"BEntID":391149,"AutID":569674,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Mohammed","Firstname":null,"Initials":"O.B.","Affiliation":"King Saud University,  Saudi Arabia","Discriminator":null,"CorporateFlag":0,"BEntID":391149,"AutID":569675,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":407951,"LibID":36,"BRefID":393363,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"407951","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":null,"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Ascaridoidea"},{"TaxTerm":"Hysterothylacium thalassini"},{"TaxTerm":"Raphidascarididae"},{"TaxTerm":"Saurida tumbil"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"941":{"SpName":"LifeWatch Species Information Backbone","SpColID":941,"ParSpColID":39,"TopParID":39,"ShortName":"LifeWatch Species Information Backbone","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"},"507":{"SpName":"World Register of Marine Species","SpColID":507,"ParSpColID":null,"TopParID":null,"ShortName":"WoRMS website","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"WoRMS website"},"915":{"SpName":"World Register of Marine Species (WoRMS) acknowledged","SpColID":915,"ParSpColID":941,"TopParID":39,"ShortName":"WoRMS ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn"},"947":{"SpName":"WoRMS ackn - direct reference","SpColID":947,"ParSpColID":915,"TopParID":39,"ShortName":"WoRMS ackn - direct","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn/WoRMS ackn - direct"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":391149,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":391149,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":391149,"AbstractFlag":1,"LangID":44,"LangCode":"pt","Lang":"Portuguese","DutchTerm":"Portugees","LangCodeExtended":"por"}],"urls":[{"URL":"https://dx.doi.org/10.1590/1678-4162-13206","externalID":"10.1590/1678-4162-13206","URLTypeCode":"DOI","URLID":133286,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Ascaridoidea","AphiaID":22816,"TaxtID":55789},{"TaxTerm":"Hysterothylacium thalassini","AphiaID":993904,"TaxtID":55791},{"TaxTerm":"Raphidascarididae","AphiaID":731849,"TaxtID":42797},{"TaxTerm":"Saurida tumbil","AphiaID":217663,"TaxtID":55790}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Ascaridoidea","DutchTerm":null,"AphiaID":22816,"TaxtID":55789},{"TaxTerm":"Hysterothylacium thalassini","DutchTerm":null,"AphiaID":993904,"TaxtID":55791},{"TaxTerm":"Raphidascarididae","DutchTerm":null,"AphiaID":731849,"TaxtID":42797},{"TaxTerm":"Saurida tumbil","DutchTerm":null,"AphiaID":217663,"TaxtID":55790}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"VLIZ2000\\robbe.vanhoecke","newSesDate":{"date":"2024-07-22 09:13:51.583000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"VLIZ2000\\robbe.vanhoecke","updSesDate":{"date":"2024-07-22 09:13:51.583000","timezone_type":3,"timezone":"Europe/Brussels"}}}