{"refrec":{"BRefID":395667,"RR":"<b>Granada, L.; Morão, I.F.C.; Lemos, M.F.L.; Bossier, P.; Novais, S.C.</b> (2025). Molecular mechanisms of induced thermotolerance in two strains of <i>Brachionus koreanus</i> (Rotifera: Monogononta). <i>Aquaculture 595</i>: 741125. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2024.741125\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2024.741125</a>","BEntID":393455,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture 595</i>: 741125. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2024.741125\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2024.741125</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Granada, L.; Morão, I.F.C.; Lemos, M.F.L.; Bossier, P.; Novais, S.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Granada, L. <i>et al.</i>","Englishabstract":"Temperature plays an important role in the occurrence and performance of organisms in aquatic ecosystems and is also one of the main environmental factors affecting species' survival and growth in aquaculture. As an important species for aquaculture sustainability, the rotifer <i>Brachionus</i> sp. benefits from inducible phenotypic traits that allow the organisms to cope with environmental stress. The exposure to high temperature has shown to increase production of heat shock proteins (HSPs) and histone modifications in several organisms, resulting in induced thermotolerance. This study aimed to evaluate the potential of non-lethal heat shock (NLHS) to induce thermotolerance in two strains of <i>B. koreanus</i> and pinpoint some of the molecular mechanisms involved in the process. Exposure of organisms to 42&nbsp;°C for 30&nbsp;min, with a subsequent recovery at 25&nbsp;°C for 8&nbsp;h demonstrated to increase thermotolerance in up to three-fold when organisms were, in a posterior phase, subjected to a lethal temperature. This study also showed that one of the strains is tendentially more thermotolerant than the other. Indeed, NLHS exposure resulted in increased mRNA expression of different <i>Hsp</i> genes and production of HSP70 in general, but different patterns of expression were observed between strains. However, a single NLHS showed to have no effects in epigenetic mechanisms, suggesting that the induced capacity to tolerate heat stress was transient and some more cycles of NLHS may be needed to promote a persistent effect.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Molecular mechanisms of induced thermotolerance in two strains of <i>Brachionus koreanus</i> (Rotifera: Monogononta)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-09-25 01:36:50.712317","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Epigenetic modifications; Heat shock proteins; Induced thermotolerance; Non-lethal heat shock; qPCR; Western blot","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-09-18","DateCreate":"2024-09-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001299838200001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.aquaculture.2024.741125"},"refs":null,"anarec":{"AnaID":395667,"PubliDate":2025,"Pagination":"741125","XtraPublOfAnaID":null,"ISBN":null,"Volume":"595","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo.  ISSN 0044-8486; e-ISSN 1873-5622","StandardTitleSer":"Aquaculture","ISSN":"0044-8486","AbbrevSer":"Aquaculture","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Granada","Firstname":"Luana","Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":393455,"AutID":368119,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-1382-9243","PersID":37240,"InsID":null},{"AutName":"Morão","Firstname":"Inês","Initials":"I.F.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":393455,"AutID":474088,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lemos","Firstname":"Marco","Initials":"M.F.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":393455,"AutID":378454,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bossier","Firstname":"Peter","Initials":"P.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":393455,"AutID":446512,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22},{"AutName":"Novais","Firstname":"Sara","Initials":"S.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":393455,"AutID":242995,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":409015,"LibID":36,"BRefID":395667,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"409015","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam; London; New York; Oxford; Tokyo"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Brachionus koreanus"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":393455,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":393455,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquaculture.2024.741125","externalID":"10.1016/j.aquaculture.2024.741125","URLTypeCode":"DOI","URLID":134794,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Brachionus koreanus","AphiaID":null,"TaxtID":54968}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Brachionus koreanus","DutchTerm":null,"AphiaID":null,"TaxtID":54968}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2024-09-16 08:02:07.323000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2025-09-18 16:44:43.870000","timezone_type":3,"timezone":"Europe/Brussels"}}}