{"refrec":{"BRefID":396297,"RR":"<b>Greening, C.; Cabotaje, P.R.; Valentin A., L.E.; Leung, P.M.; Land, H.; Rodrigues-Oliveira, T.; Ponce-Toledo, R.I.; Senger, M.; Klamke, M.A.; Milton, M.; Lappan, R.; Mullen, S.; West-Roberts, J.; Mao, J.; Song, J.; Schoelmerich, M.; Stairs, C.W.; Schleper, C.; Grinter, R.; Spang, A.; Banfield, J.F.; Berggren, G.</b> (2024). Minimal and hybrid hydrogenases are active from archaea. <i>Cell 187(13)</i>: 3357-3372.e19. <a href=\"https://dx.doi.org/10.1016/j.cell.2024.05.032\" target=\"_blank\">https://dx.doi.org/10.1016/j.cell.2024.05.032</a>","BEntID":394085,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Cell 187(13)</i>: 3357-3372.e19. <a href=\"https://dx.doi.org/10.1016/j.cell.2024.05.032\" target=\"_blank\">https://dx.doi.org/10.1016/j.cell.2024.05.032</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Greening, C.; Cabotaje, P.R.; Valentin A., L.E.; Leung, P.M.; Land, H.; Rodrigues-Oliveira, T.; Ponce-Toledo, R.I.; Senger, M.; Klamke, M.A.; Milton, M.; Lappan, R.; Mullen, S.; West-Roberts, J.; Mao, J.; Song, J.; Schoelmerich, M.; Stairs, C.W.; Schleper, C.; Grinter, R.; Spang, A.; Banfield, J.F.; Berggren, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Greening, C. <i>et al.</i>","Englishabstract":"<span style=\"background-color:rgb(255,255,255);color:rgb(46,46,46);\">Microbial hydrogen (H</span><sub>2</sub><span style=\"background-color:rgb(255,255,255);color:rgb(46,46,46);\">) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H</span><sub>2</sub><span style=\"background-color:rgb(255,255,255);color:rgb(46,46,46);\">-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H</span><sub>2</sub><span style=\"background-color:rgb(255,255,255);color:rgb(46,46,46);\">. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H</span><sub>2</sub><span style=\"background-color:rgb(255,255,255);color:rgb(46,46,46);\"> catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H</span><sub>2</sub><span style=\"background-color:rgb(255,255,255);color:rgb(46,46,46);\">-metabolizing enzymes.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Minimal and hybrid hydrogenases are active from archaea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"archaea; hydrogen; hydrogenase; anaerobic; eukaryogenesis","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-10-16","DateCreate":"2024-10-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.cell.2024.05.032"},"refs":null,"anarec":{"AnaID":396297,"PubliDate":2024,"Pagination":"3357-3372.e19","XtraPublOfAnaID":null,"ISBN":null,"Volume":"187","Issue":"13","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":238921,"SerRR":"Cell. Cell Press: Cambridge.  ISSN 0092-8674; e-ISSN 1097-4172","StandardTitleSer":"Cell","ISSN":"0092-8674","AbbrevSer":"Cell","StandardTitleMon":null,"StartPage":3357,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Spang","Firstname":"Anja","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":394085,"AutID":320546,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-6518-8556","PersID":35631,"InsID":13655}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":394085,"BEntPrivate":null,"availability":[{"BInstID":409662,"LibID":2779,"BRefID":396297,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"409662","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":21,"AccessConstraint":"Attribution (CC BY)","LicURL":"https://creativecommons.org/licenses/by/4.0/"}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Cell Press","City":"Cambridge"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":394085,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":394085,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.cell.2024.05.032","externalID":"10.1016/j.cell.2024.05.032","URLTypeCode":"DOI","URLID":135898,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"daan.van.loon@nioz.nl","newSesDate":{"date":"2024-10-16 11:41:18.170000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"daan.van.loon@nioz.nl","updSesDate":{"date":"2024-10-16 11:41:18.170000","timezone_type":3,"timezone":"Europe/Brussels"}}}