{"refrec":{"BRefID":396343,"RR":"<b>Mendonça, I.R.W.; Theirlynck, T.; Zettler, E.R.; Amaral-Zettler, L.A.; Oliveira, M.C.</b> (2024). Microbiome changes in a stranding simulation of the holopelagic macroalgae Sargassum natans and Sargassum fluitans. <i>Ocean and Coastal Research 72</i>: e24037. <a href=\"https://dx.doi.org/10.1590/2675-2824072.23111\" target=\"_blank\">https://dx.doi.org/10.1590/2675-2824072.23111</a>","BEntID":394131,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Ocean and Coastal Research 72</i>: e24037. <a href=\"https://dx.doi.org/10.1590/2675-2824072.23111\" target=\"_blank\">https://dx.doi.org/10.1590/2675-2824072.23111</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Mendonça, I.R.W.; Theirlynck, T.; Zettler, E.R.; Amaral-Zettler, L.A.; Oliveira, M.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Mendonça, I.R.W. <i>et al.</i>","Englishabstract":"<span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\">Holopelagic </span><i>Sargassum</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> has been causing massive strandings on tropical Atlantic Ocean shorelines. Describing the microbiome associated with </span><i>Sargassum</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> and how it changes after stranding is important to identify potential microbial introductions to coastal environments, as well as sources of potential biotechnological resources. In this study, stranding simulation exploratory experiments were conducted for </span><i>S. fluitans</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> III and </span><i>S. natans</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> VIII on shipboard with minimum external influence. Samples for microbiome identification were collected just after removing healthy </span><i>Sargassum</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> from the seawater (0 hr) and after 24 and 48 hrs of stranding simulation under environmental conditions. The bacterial community was identified by sequencing 16S rRNA gene V3-V4 hypervariable regions, generating a total of 1,565 Amplicon Sequence Variants (ASVs). Of those, 588 were shared between </span><i>Sargassum</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> species and only 25 persisted throughout the stranding. Stranding also changed the dominance of Microtrichales and Rhodobacterales orders at 0 hr to Alteromonadales and Vibrionales after 24 hrs of exposure, the latter representing up to 92% of the relative abundance in the bacterial community. The increase in Vibrionales reinforces the need to monitor stranding sites for any potential pathogenic bacteria. At the functional level, phototrophs were the main group at 0 hr, shifting to chemoheterotrophs and fermentation within the first 24 hrs of </span><i>Sargassum</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> exposure to air conditions. The fermentative groups native to </span><i>Sargassum</i><span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\"> use stranded biomass as substrate for growth, and therefore constitute the bacteria with higher biotechnological potential.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Microbiome changes in a stranding simulation of the holopelagic macroalgae Sargassum natans and Sargassum fluitans","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<span style=\"background-color:rgb(255,255,255);color:rgb(64,61,57);\">Brown tide; Microbial community; Dysbiosis; High-throughput sequencing; Amplicon Sequence Variants</span>","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-10-21","DateCreate":"2024-10-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1590/2675-2824072.23111"},"refs":null,"anarec":{"AnaID":396343,"PubliDate":2024,"Pagination":"e24037","XtraPublOfAnaID":null,"ISBN":null,"Volume":"72","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":327924,"SerRR":"Ocean and Coastal Research. Universidade de Sâo Paulo. Instituto Oceanográfico: São Paulo.  e-ISSN 2675-2824","StandardTitleSer":"Ocean and Coastal Research","ISSN":null,"AbbrevSer":null,"StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Mendonça","Firstname":"Inara","Initials":"I.R.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":394131,"AutID":514525,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Theirlynck","Firstname":"Tom","Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":394131,"AutID":514524,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0001-9946-8479","PersID":42028,"InsID":13655},{"AutName":"Zettler","Firstname":"Erik","Initials":"E.R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":394131,"AutID":398631,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-9266-1142","PersID":32745,"InsID":13655},{"AutName":"Amaral-Zettler","Firstname":"Linda","Initials":"L.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":394131,"AutID":398632,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":9507,"InsID":13655},{"AutName":"Oliveira","Firstname":"Mariana Cabral","Initials":"M.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":394131,"AutID":470751,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":394131,"BEntPrivate":null,"availability":[{"BInstID":409705,"LibID":2779,"BRefID":396343,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"409705","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":21,"AccessConstraint":"Attribution (CC BY)","LicURL":"https://creativecommons.org/licenses/by/4.0/"}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Universidade de Sâo Paulo. 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