{"refrec":{"BRefID":396515,"RR":"<b>Elkattan, A.; Matsumoto, M.; Nagata, M.; Mittraphab, Y.; Wahab, G.A.; Ashour, A.; Zaki, A.A.; Hamed, E.A.E.; Shimizu, K.</b> (2024). Isolation of holostane-type saponins from the black sea cucumber <i>Holothuria atra</i> and evaluating their anti-allergic activity: in vitro and in silico study. <i>Cytotechnology 76(6)</i>: 721-734. <a href=\"https://dx.doi.org/10.1007/s10616-024-00649-8\" target=\"_blank\">https://dx.doi.org/10.1007/s10616-024-00649-8</a>","BEntID":394303,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Cytotechnology 76(6)</i>: 721-734. <a href=\"https://dx.doi.org/10.1007/s10616-024-00649-8\" target=\"_blank\">https://dx.doi.org/10.1007/s10616-024-00649-8</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Elkattan, A.; Matsumoto, M.; Nagata, M.; Mittraphab, Y.; Wahab, G.A.; Ashour, A.; Zaki, A.A.; Hamed, E.A.E.; Shimizu, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Elkattan, A. <i>et al.</i>","Englishabstract":"Sea cucumbers are both versatile marine organisms and an Asian marine food known to have several medicinal efects. We evaluated the anti-aller?gic potential of some major purifed holostane-type saponins from the body wall of the black sea cucum?ber,<i> Holothuria atra</i>. Six saponin compounds were isolated, holothurin B (1), holothurin A (2), 24-dehy?dro echinoside A (3), desholothurin A1 (4), desholo?thurin A (5), and des 24-dehydro echinoside A (6). The structures were identifed based on spectroscopic methods and by comparison with the literature. Each compound’s inhibitory activity toward the release of β-hexosaminidase was evaluated. Among the six compounds, holothurin B (1) showed the strongest inhibition of the degranulation at all tested concen?trations in a dose-dependent manner, compared to the positive control, quercetin. We also observed that holothurin B (1) was able to alleviate the infamma?tory mediators interleukin (IL)-6, IL-13, and tumor necrosis factor-alpha (TNF-α). Holothurin B (1) also inhibited the Ca2+ infux stimulated by the cal?cium ionophore A23187, by suppressing the expres?sion of inositol-1,4,5-triphosphate receptor (IP3R) mRNA. These results suggest that (i) holothurin B (1) has good anti-allergy activity without cytotoxic?ity at efective concentrations, and (ii) this compound could be a lead compound for the treatment of aller?gic diseases and associated infammation. We also performed a molecular docking study for the tested compounds to correlate their binding modes and afnity for the IP3R with the in&nbsp; vitro results. The results concluded that the holostane-type saponins could be used as anti-allergy agents, which may be attributed to their holostane group.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Isolation of holostane-type saponins from the black sea cucumber <i>Holothuria atra</i> and evaluating their anti-allergic activity: in vitro and in silico study","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<i>Holothuria atra</i>,  Holothuroidea,  holostane-type saponin,  ß-hexosaminidase, anti-allergy","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-11-06","DateCreate":"2024-11-06","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10616-024-00649-8"},"refs":null,"anarec":{"AnaID":396515,"PubliDate":2024,"Pagination":"721-734","XtraPublOfAnaID":null,"ISBN":null,"Volume":"76","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45694,"SerRR":"Cytotechnology. 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