Document of bibliographic reference 396745

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

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AS

Title

Complete mitochondrial genome and phylogenetic analysis of Dollfustrema vaneyi (Trematoda: Bucephalidae)

Abstract

Background The Bucephalidae is a large family of digenean trematodes but most previous analyses of its phyloge?netic position have relied on a single mitochondrial gene or morphological features. Mitochondrial genomes (mitog?enomes) remain unavailable for the entire family. To address this, we sequenced the complete mitogenome of Doll?fustrema vaneyi and analyzed the phylogenetic relationships with other trematodes. Results The circular genome of Dollfustrema vaneyi spanned 14,959 bp and contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a major non-coding region. We used concatenated amino acid and nucleotide sequences of all 36 genes for phylogenetic analyses, conducted using MrBayes, IQ-TREE and Phy?loBayes. We identifed pronounced topological instability across diferent analyses. The addition of recently sequenced two mitogenomes for the Aspidogastrea subclass along with the use of a site-heterogeneous model stabilized the topology, particularly the positions of Azygiidae and Bucephalidae. The stabilized results indicated that Azygii?dae was the closest lineage to Bucephalidae in the available dataset, and together, they clustered at the base of the Plagiorchiida. Conclusions Our study provides the frst comprehensive description and annotation of the mitochondrial genome for the Bucephalidae family. The results indicate a close phylogenetic relationship between Azygiidae and Bucephali?dae, and reveal their basal placement within the order Plagiorchiida. Furthermore, the inclusion of Aspidogastrea mitogenomes and the site-heterogeneous model signifcantly improved the topological stability. These data will provide key molecular resources for future taxonomic and phylogenetic studies of the family Bucephalidae. 

Bibliographic citation

Hu, Y.; Ye, T.; Zou, H.; Wang, G.-T.; Li, W.-X.; Zhang, D. (2024). Complete mitochondrial genome and phylogenetic analysis of Dollfustrema vaneyi (Trematoda: Bucephalidae). BMC Genom. 25(1). https://dx.doi.org/10.1186/s12864-024-10740-1

Is peer reviewed

true

Access rights

open access

Is accessible for free

true