{"refrec":{"BRefID":39937,"RR":"<b>Chin, G.J.; Payza, K.; Price, D.A.; Greenberg, M.J.; Doble, K.E.</b> (1994). Characterization and solubilization of the FMRFamide receptor of squid. <i>Biol. Bull. 187(2)</i>: 185-199","BEntID":39937,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Biol. Bull. 187(2)</i>: 185-199","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Chin, G.J.; Payza, K.; Price, D.A.; Greenberg, M.J.; Doble, K.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Chin, G.J. <i>et al.</i>","Englishabstract":"The optic lobe of squid (<i>Loligo pealei</i>) contains FMRFamide receptors that can bind an iodinated FMRFamide analog: [125I]-desaminoTyr-Phe- norLeu-Arg-Phe-amide ([125I]-daYFnLRFa). Radioligand binding assays revealed that squid FMRFamide receptors are specific, saturable, high affinity sites (Kd = 0.15 nM) densely concentrated in optic lobe membranes (Bmax = 237 fmole/mg protein). The receptors appeared to be coupled to Gs because guanine nucleotides inhibit receptor binding and the stimulation of adenylate cyclase by FMRFamide is GTP-dependent. Both the binding and cyclase data showed that FMRFamide, but not FMRF-OH, interacts at FMRFamide receptors; thus the C-terminal Arg-Phe-amide is critical for binding. The high binding affinity of FMRFamide (0.4 nM IC50) was specific for FMRFamide-like peptides. The structure-activity relations of many FMRFamide analogs were defined in detail and were nearly identical for both the membrane-bound and detergent-solubilized receptors. We also found that squid optic lobe contains FMRFamide-like reactivity as measured with both a radioimmunoassay and a radioreceptor assay. Moreover, we have sequenced a fragment of genomic DNA that encodes a FMRFamide precursor. Our findings in sum suggest that FMRFamide is a neurotransmitter in squid optic lobe, and that this tissue is a good source from which to purify FMRFamide receptors.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization and solubilization of the FMRFamide receptor of squid","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1994,"MonPub":null,"DateUpdate":"2003-09-23","DateCreate":"2003-09-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1994PR25500008","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":39937,"PubliDate":1994,"Pagination":"185-199","XtraPublOfAnaID":null,"ISBN":null,"Volume":"187","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42437,"SerRR":"The Biological Bulletin. 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