{"refrec":{"BRefID":40165,"RR":"<b>Browne, C.L.; Creton, R.; Karplus, E.; Mohler, P.J.; Palazzo, R.E.; Miller, A.L.</b> (1996). Analysis of the calcium transient at NEB during the first cell cycle in dividing sea urchin eggs. <i>Biol. Bull. 191(1)</i>: 5-16","BEntID":40165,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Biol. Bull. 191(1)</i>: 5-16","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Browne, C.L.; Creton, R.; Karplus, E.; Mohler, P.J.; Palazzo, R.E.; Miller, A.L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Browne, C.L. <i>et al.</i>","Englishabstract":"Accumulating evidence from several systems suggests that nuclear envelope breakdown (NEB) is triggered by an endogenous transient of free calcium. Using h- and f-semisynthetic aequorins as cytosolic calcium indicators, we have clearly and regularly visualized a single large, global calcium transient just before first NEB in normally developing, monospermic <i>Lytechinus</i> eggs. Although similar transients were not observed at NEB in subsequent cell cycles, microinjection of the calcium buffer BAPTA into one blastomere of the two-celled embryo resulted in the inhibition of NEB. The NEB transient in the first cell cycle was some five-fold smaller than the one associated with egg activation. Our data suggest that this transient takes the form of a calcium wave that spreads inwards from the periphery of the egg toward the nucleus. We confirmed that these NEB transients did not require extracellular Ca2+. In polyspermic eggs, NEB-associated transients were four-fold larger than in monospermic eggs and were periodically repeated. Examination of the distribution of fluorescein-conjugated aequorins with a laser scanning confocal microscope indicated that aequorin both enters the nucleus and is evenly distributed within the cytosol of the egg. The use of h- and f-aequorins did not reveal any NEB transients during subsequent cell cycles, nor did we detect transients associated with other cell cycle events. However, a complex train of calcium transients in the form of both localized pulses and propagated waves was detected from embryos beginning at about the morula-to-blastula transition and continuing through to hatching.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Analysis of the calcium transient at NEB during the first cell cycle in dividing sea urchin eggs","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1996,"MonPub":null,"DateUpdate":"2003-09-24","DateCreate":"2003-09-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1996VE29500002","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":40165,"PubliDate":1996,"Pagination":"5-16","XtraPublOfAnaID":null,"ISBN":null,"Volume":"191","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42437,"SerRR":"The Biological Bulletin. 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