{"refrec":{"BRefID":4020,"RR":"<b>Huo, J.-Z.; Nelis, H.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P.</b> (1999). Simultaneous determination of a-tocopheryl acetate and tocopherols in aquatic organisms and fish feed. <i>J. Chromatogr. B(724)</i>: 249-255","BEntID":4020,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Chromatogr. B(724)</i>: 249-255","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Huo, J.-Z.; Nelis, H.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Huo, J.-Z. <i>et al.</i>","Englishabstract":"In aquaculture, alpha-tocopheryl acetate (alpha-TA) is the main source of vitamin E used to fortify fish feed. Alpha-TA in fish is often determined indirectly, i.e. by alkaline hydrolysis, followed by quantitation of ‘total alpha-tocopherol’ (alpha-T) and substraction of the natively present alpha-T. The aim of this study was to develop an HPLC method for the simultaneous quantitative determination of alpha-TA and free tocopherols in aquatic organisms and fish feed. The assay consists of a simple extraction with methanol containing butylhydroxytoluene (BHT) as an antioxidant, followed by reversed-phase chromatography with consecutive UV and fluorescence detection of alpha-TA and tocopherols, respectively. The peak of the internal standard tocol in the fluorescence trace was used for quantitation. Linearity was achieved over the range of 0.2 to 4.2 µg alpha-TA per ml extract of <i>Artemia</i> nauplii, which would correspond to 30.7 to 614.4 µg/g dry mass. The within-run coefficient of variation was 1.9% at a level of 310 µg/g dry mass. The recovery of alpha-TA ranged from 97.7 to 100.8% (concentration=2.1 and 20.5 µg/ml, <i>n</i>=6). The detection limit was about 7 ng and the quantification limit on spiked samples was 0.2 µg/ml. This method was routinely applied to determine alpha-TA and alpha-, gamma- and delta-tocopherol (alpha-T, gamma-T, delta-T) simultaneously in <i>Artemia</i> , fish feed, shrimp eggs and various other aquatic organisms.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Simultaneous determination of a-tocopheryl acetate and tocopherols in aquatic organisms and fish feed","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"Vitamins; alpha-Tocopheryl acetate; Tocopherol","Notes":null,"AnaPub":1999,"MonPub":null,"DateUpdate":"2015-07-13","DateCreate":"2000-10-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":4020,"PubliDate":1999,"Pagination":"249-255","XtraPublOfAnaID":null,"ISBN":null,"Volume":"B","Issue":"724","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43215,"SerRR":"Journal of Chromatography A. 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