{"refrec":{"BRefID":4037,"RR":"<b>Vandenberghe, J.; Verdonck, L.; Robles-Arozarena, R.; Rivera, G.; Bolland, A.; Balladares, M.; Gomez-Gil, B.; Calderon, J.; Sorgeloos, P.; Swings, J.</b> (1999). Vibrios associated with <i>Litopenaeus vannamei</i> larvae, postlarvae, broodstock, and hatchery probionts. <i>Appl. Environ. Microbiol. 65(6)</i>: 2592-2597","BEntID":4037,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Appl. Environ. Microbiol. 65(6)</i>: 2592-2597","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vandenberghe, J.; Verdonck, L.; Robles-Arozarena, R.; Rivera, G.; Bolland, A.; Balladares, M.; Gomez-Gil, B.; Calderon, J.; Sorgeloos, P.; Swings, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vandenberghe, J. <i>et al.</i>","Englishabstract":"Several bacteriological surveys were performed from 1994 to 1996 at different <i>Litopenaeus vannamei</i> hatcheries (in Ecuador) and shrimp farms (in Mexico). Samples were taken from routine productions of healthy and diseased <i>L. vannamei</i> larvae, postlarvae, and their culture environment and from healthy and diseased juveniles and broodstock. In Ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been determined. Strains were characterized by Biolog metabolic fingerprinting and identified by comparison to a database of 850 <i>Vibrio</i> type and reference strains. A selection of strains was further genotypically fine typed by AFLP. <i>Vibrio alginolyticus</i> is predominantly present in all larval stages and is associated with healthy nauplius and zoea stages. AFLP genetic fingerprinting shows high genetic heterogeneity among <i>V. alginolyticus</i> strains, and the results suggest that putative probiotic and pathogenic strains each have specific genotypes. <i>V. alginolyticus</i> was found to be associated with larvae with the zoea 2 syndrome and the mysis mold syndrome, while different <i>Vibrio</i> species (<i>V. alginolyticus</i> and <i>V. harveyi</i>) are associated with the bolitas syndrome. <i>V. harveyi</i> is associated with diseased postlarvae, juveniles, and broodstock. The identities of the strains identified as <i>V. harveyi</i> by the Biolog system could not be unambiguously confirmed by AFLP genomic fingerprinting. <i>Vibrio</i> strain STD3-988 and one unidentified strain (STD3-959) are suspected pathogens of only juvenile and adult stages. <i>V. parahaemolyticus, Photobacterium damselae</i>, and <i>V. mimicus</i> are associated with juvenile and adult stages.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Vibrios associated with <i>Litopenaeus vannamei</i> larvae, postlarvae, broodstock, and hatchery probionts","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:33:14.980000","timezone_type":1,"timezone":"+00:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"Larvae; Community structure; Biotyping; Genotyping; Fingerprinting; Ecuador; Mexico; Shrimp culture; Pathogenic bacteria; Chemotaxonomy; Genotypes; <i>Litopenaeus vannamei; Vibrio alginolyticus; Vibrio harveyi; Vibrio parahaemolyticus; Photobacterium damsela; Vibrio mimicus; Photobacterium damselae</i>; ISE, Ecuador","Notes":null,"AnaPub":1999,"MonPub":null,"DateUpdate":"2000-10-30","DateCreate":"2000-10-30","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000080624300047","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":4037,"PubliDate":1999,"Pagination":"2592-2597","XtraPublOfAnaID":null,"ISBN":null,"Volume":"65","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42360,"SerRR":"Applied and Environmental Microbiology. 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