{"refrec":{"BRefID":404456,"RR":"<b>Pradel, N.; Bartoli, M.; Koenen, M.; Bale, N.; Neumann-Schaal, M.; Spröer, C.; Bunk, B.; Rohde, M.; Pester, M.; Spring, S.</b> (2024). Description and genome analysis of a novel archaeon isolated from a syntrophic pyrite-forming enrichment culture and reclassification of <i>Methanospirillum hungatei</i> strains GP1 and SK as <i>Methanospirillum purgamenti </i>sp. nov. <i>PLoS One 19(8)</i>: e0308405. <a href=\"https://dx.doi.org/10.1371/journal.pone.0308405\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0308405</a>","BEntID":402247,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":" <i>PLoS One 19(8)</i>: e0308405. <a href=\"https://dx.doi.org/10.1371/journal.pone.0308405\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0308405</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Pradel, N.; Bartoli, M.; Koenen, M.; Bale, N.; Neumann-Schaal, M.; Spröer, C.; Bunk, B.; Rohde, M.; Pester, M.; Spring, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Pradel, N. <i>et al.</i>","Englishabstract":"<span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">The archaeal isolate J.3.6.1-F.2.7.3</span><sup>T</sup><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> was obtained from an anaerobic enrichment culture, where it may play an important role in methane production during pyrite formation. The new isolate formed a species-level clade with </span><i>Methanospirillum hungatei</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> strains GP1 and SK, which is separate from the type strain JF-1</span><sup>T</sup><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">. Cultivation-independent surveys indicate the occurrence of this phylogenetic group in sediments and anaerobic digesters. The abundance of this clade appears to be negatively affected by high nitrogen loads, indicating a sensitivity to certain nitrogen compounds that is not known in </span><i>M</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">. </span><i>hungatei</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> JF-1</span><sup>T</sup><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">. The relatively large core genome of this </span><i>Methanospirillum</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> clade is indicative of niche specialization and efficient control of horizontal gene transfer. Genes for nitrogenase and F</span><sub>420</sub><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">-dependent secondary alcohol dehydrogenase contribute to the metabolic versatility of this lineage. Characteristics of the new isolate such as the ability to utilize 2-propanol as an electron donor or the requirement for acetate as a carbon source are found also in the strains GP1 and SK, but not in the type strain </span><i>M</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">. </span><i>hungatei</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> JF-1</span><sup>T</sup><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">. Based on the genomic differences to related species, a new species within the genus </span><i>Methanospirillum</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> is proposed with the name </span><i>M</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\">. </span><i>purgamenti</i><span style=\"background-color:rgb(255,255,255);color:rgb(32,32,32);\"> sp. nov. The determined phenotypic characteristics support this proposal and indicate a metabolic adaptation to a separate ecological niche.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Description and genome analysis of a novel archaeon isolated from a syntrophic pyrite-forming enrichment culture and reclassification of <i>Methanospirillum hungatei</i> strains GP1 and SK as <i>Methanospirillum purgamenti </i>sp. nov.","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Genomics; Ribosomal RNA; Formates; Phylogenetic analysis; Phylogenetics; Lipids; Methanogens; Glycerol","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-11-26","DateCreate":"2024-11-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0308405"},"refs":null,"anarec":{"AnaID":404456,"PubliDate":2024,"Pagination":"e0308405","XtraPublOfAnaID":null,"ISBN":null,"Volume":"19","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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