{"refrec":{"BRefID":404797,"RR":"<b>Sorokin, D.Y.; Merkel, A.Y.; Kolganova, T.V.; Bale, N.; Sinninghe Damsté, J.S</b> (2024). Natronospira bacteriovora sp. nov., and Natronospira elongata sp. nov., extremely salt-tolerant predatory proteolytic bacteria from soda lakes and proposal to classify the genus Natronospira into Natronospiraceae fam. nov., and Natronospirales ord. nov., within the class Gammaproteobacteria. <i>Syst. Appl. Microbiol. 47(4)</i>: 126519. <a href=\"https://dx.doi.org/10.1016/j.syapm.2024.126519\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2024.126519</a>","BEntID":402590,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Syst. Appl. Microbiol. 47(4)</i>: 126519. <a href=\"https://dx.doi.org/10.1016/j.syapm.2024.126519\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2024.126519</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sorokin, D.Y.; Merkel, A.Y.; Kolganova, T.V.; Bale, N.; Sinninghe Damsté, J.S","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sorokin, D.Y. <i>et al.</i>","Englishabstract":"<span style=\"color:rgb(31,31,31);\">The genus </span><i>Natronospira</i><span style=\"color:rgb(31,31,31);\"> is represented by a single species of extremely salt-tolerant aerobic alkaliphilic </span><a href=\"https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/proteolytic-bacteria\"><span style=\"color:rgb(31,31,31);\">proteolytic bacterium</span></a><span style=\"color:rgb(31,31,31);\">, isolated from hypersaline soda lakes. When cells of Gram-positive </span><a href=\"https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/coccus\"><span style=\"color:rgb(31,31,31);\">cocci</span></a><span style=\"color:rgb(31,31,31);\"> were used as a substrate instead of proteins at extremely haloalkaline conditions, two new members of this genus were enriched and isolated in pure culture from the same sites. Strains AB-CW1 and AB-CW4 are obligate aerobic heterotrophic proteolytic bacteria able to feed on both live and dead cells of staphylococci and a range of </span><a href=\"https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/peptides-and-proteins\"><span style=\"color:rgb(31,31,31);\">proteins and peptides</span></a><span style=\"color:rgb(31,31,31);\">. Similar to the type species, </span><i>N. proteinivora</i><span style=\"color:rgb(31,31,31);\">, the isolates are extremely salt-tolerant obligate </span><a href=\"https://www.sciencedirect.com/topics/immunology-and-microbiology/alkaliphile\"><span style=\"color:rgb(31,31,31);\">alkaliphiles</span></a><span style=\"color:rgb(31,31,31);\">. However, </span><i>N. proteinivora</i><span style=\"color:rgb(31,31,31);\"> was unable to use </span><a href=\"https://www.sciencedirect.com/topics/immunology-and-microbiology/bacterial-cell\"><span style=\"color:rgb(31,31,31);\">bacterial cells</span></a><span style=\"color:rgb(31,31,31);\"> as a substrate. </span><a href=\"https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/electron-microscopy\"><span style=\"color:rgb(31,31,31);\">Electron microscopy</span></a><span style=\"color:rgb(31,31,31);\"> showed direct contact between the prey and predator cells. Functional analysis of the AB-CW1 and AB-CW4 genomes identified two sets of genes coding for extracellular </span><a href=\"https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/enzyme\"><span style=\"color:rgb(31,31,31);\">enzymes</span></a><span style=\"color:rgb(31,31,31);\"> potentially involved in the predation and </span><a href=\"https://www.sciencedirect.com/topics/immunology-and-microbiology/protein-degradation\"><span style=\"color:rgb(31,31,31);\">proteolysis</span></a><span style=\"color:rgb(31,31,31);\">, respectively. The first set includes several copies of lysozyme-like GH23 peptidoglycan-lyase and murein-specific M23 [Zn]-di-peptidase enabling the cell wall degradation. The second set features multiple copies of secreted </span><a href=\"https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/serine\"><span style=\"color:rgb(31,31,31);\">serine</span></a><span style=\"color:rgb(31,31,31);\"> and metallopeptidases apparently allowing for the strong proteolytic phenotype. </span><a href=\"https://www.sciencedirect.com/topics/immunology-and-microbiology/phylogenomics\"><span style=\"color:rgb(31,31,31);\">Phylogenomic</span></a><span style=\"color:rgb(31,31,31);\"> analysis placed the isolates into the genus </span><i>Natronospira</i><span style=\"color:rgb(31,31,31);\"> as two novel species members, and furthermore indicated that this genus forms a deep-branching </span><a href=\"https://www.sciencedirect.com/topics/immunology-and-microbiology/lineages\"><span style=\"color:rgb(31,31,31);\">lineage</span></a><span style=\"color:rgb(31,31,31);\"> of a new family (</span><i>Natronospiraceae</i><span style=\"color:rgb(31,31,31);\">) and order (</span><i>Natronospirales</i><span style=\"color:rgb(31,31,31);\">) within the class </span><a href=\"https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/gammaproteobacteria\"><span style=\"color:rgb(31,31,31);\">Gammaproteobacteria</span></a><span style=\"color:rgb(31,31,31);\"><i>.</i> On the basis of distinct phenotypic and genomic properties, strain AB-CW1</span><sup>T</sup><span style=\"color:rgb(31,31,31);\"> (JCM 335396&nbsp;=&nbsp;UQM 41579) is proposed to be classified as </span><i>Natronospira elongata</i><span style=\"color:rgb(31,31,31);\"> sp. nov., and AB-CW4</span><sup>T</sup><span style=\"color:rgb(31,31,31);\"> (JCM 335397&nbsp;=&nbsp;UQM 41580) as </span><i>Natronospira bacteriovora</i><span style=\"color:rgb(31,31,31);\"> sp. nov.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Natronospira bacteriovora sp. nov., and Natronospira elongata sp. nov., extremely salt-tolerant predatory proteolytic bacteria from soda lakes and proposal to classify the genus Natronospira into Natronospiraceae fam. nov., and Natronospirales ord. nov., within the class Gammaproteobacteria","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-06 01:35:24.948481","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<p style=\"margin-left:0px;\"><i>Natronospira; </i>Predatory; Proteolytic; Haloalkaliphilic; Soda lakes","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2024-12-09","DateCreate":"2024-12-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.syapm.2024.126519"},"refs":null,"anarec":{"AnaID":404797,"PubliDate":2024,"Pagination":"126519","XtraPublOfAnaID":null,"ISBN":null,"Volume":"47","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43944,"SerRR":"Systematic and Applied Microbiology. Elsevier: Jena.  ISSN 0723-2020; e-ISSN 1618-0984","StandardTitleSer":"Systematic and Applied Microbiology","ISSN":"0723-2020","AbbrevSer":"Syst. Appl. Microbiol.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Sorokin","Firstname":"Dimitry","Initials":"D.Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":402590,"AutID":335739,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Merkel","Firstname":"Alexander","Initials":"A.Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":402590,"AutID":335714,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kolganova","Firstname":"Tatyana V.","Initials":"T.V.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":402590,"AutID":577974,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bale","Firstname":"Nicole","Initials":"N.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":402590,"AutID":258288,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":29640,"InsID":13655},{"AutName":"Sinninghe Damsté","Firstname":"Jaap","Initials":"J.S","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":402590,"AutID":324945,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-8683-1854","PersID":29942,"InsID":13655}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":402590,"BEntPrivate":null,"availability":[{"BInstID":410706,"LibID":2779,"BRefID":404797,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"410706","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":261,"Classification":"NIOZ files","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":null,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":21,"AccessConstraint":"Attribution (CC BY)","LicURL":"https://creativecommons.org/licenses/by/4.0/"}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Elsevier","City":"Jena"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":402590,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":402590,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.syapm.2024.126519","externalID":"10.1016/j.syapm.2024.126519","URLTypeCode":"DOI","URLID":138000,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"amber.van.t.veen@nioz.nl","newSesDate":{"date":"2024-12-09 08:41:48.753000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"amber.van.t.veen@nioz.nl","updSesDate":{"date":"2024-12-09 08:41:48.753000","timezone_type":3,"timezone":"Europe/Brussels"}}}