{"refrec":{"BRefID":405282,"RR":"<b>Peperzak, L.</b> (2024). The serial dilution culture-most probable number assay to estimate phytoplankton concentrations in ballast water: comments and improvements. <i>Front. Mar. Sci. 11</i>: 1494598. <a href=\"https://dx.doi.org/10.3389/fmars.2024.1494598\" target=\"_blank\">https://dx.doi.org/10.3389/fmars.2024.1494598</a>","BEntID":403077,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Mar. Sci. 11</i>: 1494598. <a href=\"https://dx.doi.org/10.3389/fmars.2024.1494598\" target=\"_blank\">https://dx.doi.org/10.3389/fmars.2024.1494598</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Peperzak, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Peperzak, L.","Englishabstract":"In ecology, the enumeration of living aquatic unicellular protists is a century-old complicated task. The organisms are small, perhaps motile, and if they are non-motile their vitality is hard to gauge. A respectable technique still in use today is the Serial Dilution Culture-Most Probable Number (MPN) assay that determines the total viable phytoplankton concentration from the highest sample dilutions that exhibit chlorophyll-fluorescence after incubation. However, 99% of extant phytoplankton species have not been shown to grow in MPN tubes (false-negatives) and in ballast water applications, 10-50 µm species can be outcompeted by &lt;10 µm autotrophs in abundance, biomass, and chlorophyll-fluorescence (false-positives). In addition, it is shown that after microscopic identification, contrary to established practice, the concentration of individual species or species-groups (10-50 µm) cannot be derived from standard MPN tables. Examples of a corrected derivation are given for simple (2-3 species) assemblages, but this correction becomes increasingly difficult in more complex mixtures. The MPN assay should not be used as a quantitative method in ecological studies or in applications such as ballast water testing.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The serial dilution culture-most probable number assay to estimate phytoplankton concentrations in ballast water: comments and improvements","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:59.824360","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"serial dilution culture-most probable number; SDC-MPN; calculation correction; false?positives; false-negatives; phytoplankton; model, ballast water","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2025-01-13","DateCreate":"2025-01-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.3389/fmars.2024.1494598"},"refs":null,"anarec":{"AnaID":405282,"PubliDate":2024,"Pagination":"1494598","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":233602,"SerRR":"Frontiers in Marine Science. 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