{"refrec":{"BRefID":405671,"RR":"<b>Stout, L.; Daffe, G.; Chambouvet, A.; Correia, S.; Culloty, S.C.; Freitas, R.; Iglesias, D.; Jensen, K.T.; Joaquim, S.; Lynch, S.; Magalhães, L.; Mahony, K.; Malham, S.K.; Matias, D.; Rocroy, M.; Thieltges, D.W.; de Montaudouin, X.</b> (2024). Morphological vs. molecular identification of trematode species infecting the edible cockle Cerastoderma edule across Europe. <i>IJP 25</i>: 101019. <a href=\"https://dx.doi.org/10.1016/j.ijppaw.2024.101019\" target=\"_blank\">https://dx.doi.org/10.1016/j.ijppaw.2024.101019</a>","BEntID":403466,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>IJP 25</i>: 101019. <a href=\"https://dx.doi.org/10.1016/j.ijppaw.2024.101019\" target=\"_blank\">https://dx.doi.org/10.1016/j.ijppaw.2024.101019</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Stout, L.; Daffe, G.; Chambouvet, A.; Correia, S.; Culloty, S.C.; Freitas, R.; Iglesias, D.; Jensen, K.T.; Joaquim, S.; Lynch, S.; Magalhães, L.; Mahony, K.; Malham, S.K.; Matias, D.; Rocroy, M.; Thieltges, D.W.; de Montaudouin, X.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Stout, L. <i>et al.</i>","Englishabstract":"<span style=\"color:rgb(31,31,31);\">Identifying marine trematode parasites in host tissue can be complicated when there is limited morphological differentiation between species infecting the same host species. This poses a challenge for regular surveys of the parasite communities in species of socio-economic and ecological importance. Our study focused on identifying digenean trematode species infecting the marine bivalve </span><i>Cerastoderma edule</i><span style=\"color:rgb(31,31,31);\"> across Europe by comparing morphological and molecular species identification methods. Cockles were sampled from ten locations to observe the trematode parasites under a stereomicroscope (morphological identification) and to isolate individuals for phylogenetic analyses using two gene markers, the small sub-unit ribosomal (18S) RNA gene (SSU rDNA) and the mitochondrial cytochrome </span><i>c</i><span style=\"color:rgb(31,31,31);\"> oxidase subunit 1 (cox1). For the first time, we compared both morphological identification and phylogenetic analyses for each of the 13 originally identified species. First, we identified a group of five species for which morphological identification matched molecular results (</span><i>Bucephalus minimus</i><span style=\"color:rgb(31,31,31);\">, </span><i>Monorchis parvus</i><span style=\"color:rgb(31,31,31);\">, </span><i>Renicola parvicaudatus</i><span style=\"color:rgb(31,31,31);\">, </span><i>Psilostomum brevicolle</i><span style=\"color:rgb(31,31,31);\">, </span><i>Himasthla interrupta</i><span style=\"color:rgb(31,31,31);\">). Second, we identified a group of six species for which molecular results revealed either misidentifications or cryptic diversity (</span><i>Gymnophallus choledochus</i><span style=\"color:rgb(31,31,31);\">, </span><i>Diphterostomum brusinae</i><span style=\"color:rgb(31,31,31);\">, </span><i>Curtuteria arguinae</i><span style=\"color:rgb(31,31,31);\">, </span><i>Himasthla quissetensis</i><span style=\"color:rgb(31,31,31);\">, </span><i>H. elongata</i><span style=\"color:rgb(31,31,31);\">, </span><i>H</i><span style=\"color:rgb(31,31,31);\">. </span><i>continua</i><span style=\"color:rgb(31,31,31);\">). Third, our analyses showed that all sequences of two expected species, </span><i>Gymnophallus minutus</i><span style=\"color:rgb(31,31,31);\"> and </span><i>G. fossarum</i><span style=\"color:rgb(31,31,31);\">, matched between the two, strongly suggesting that only </span><i>G. minutus</i><span style=\"color:rgb(31,31,31);\"> is present in the studied area. Our study clearly demonstrates that molecular tools are necessary to validate the trematode species composition. However, with 17 distinct genetic lineages detected, some of which are not fully identified, future studies are needed to clarify the identity and status (regular </span><i>vs.</i><span style=\"color:rgb(31,31,31);\"> accidental infection) of some of these cryptic trematode species.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Morphological vs. molecular identification of trematode species infecting the edible cockle Cerastoderma edule across Europe","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:59.980624","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<p style=\"margin-left:0px;\">Molecular taxonomy; Trematodes; <i>Cerastoderma edule; </i>North-East Atlantic; cox1; SSU (18S) rRNA gene","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2025-02-11","DateCreate":"2025-02-11","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.ijppaw.2024.101019"},"refs":null,"anarec":{"AnaID":405671,"PubliDate":2024,"Pagination":"101019","XtraPublOfAnaID":null,"ISBN":null,"Volume":"25","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":260431,"SerRR":"International Journal for Parasitology: Parasites and Wildlife. 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