{"refrec":{"BRefID":407945,"RR":"<b>Ruiz-Ruiz, P.; Mohedano-Caballero, P.; De Vrieze, J.</b> (2025). Ectoine production through a marine methanotroph-microalgae culture allows complete biogas valorization. <i>J. Environ. Manage. 375</i>: 124223. <a href=\"https://dx.doi.org/10.1016/j.jenvman.2025.124223\" target=\"_blank\">https://dx.doi.org/10.1016/j.jenvman.2025.124223</a>","BEntID":405746,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Environ. Manage. 375</i>: 124223. <a href=\"https://dx.doi.org/10.1016/j.jenvman.2025.124223\" target=\"_blank\">https://dx.doi.org/10.1016/j.jenvman.2025.124223</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ruiz-Ruiz, P.; Mohedano-Caballero, P.; De Vrieze, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ruiz-Ruiz, P.; Mohedano-Caballero, P.; De Vrieze, J.","Englishabstract":"Methanotrophs have recently emerged as a promising platform for producing bio-based chemicals, like ectoine, from biogas, offering an economical alternative to glucose. However, most studies have focused solely on CH<sub>4</sub> consumption, often overlooking the CO<sub>2</sub>, which is both produced by methanotrophs and present in biogas, despite its potential as a carbon source for microorganisms, such as microalgae. In this study, marine methanotrophic-microalgal cultures were enriched from environmental samples collected at the North Sea coast to explore ectoine production from both CH<sub>4</sub> and CO<sub>2</sub> in biogas. The sediment-derived culture exhibited the highest CH<sub>4</sub> removal efficiency and CO<sub>2</sub> uptake, and was selected for further experiments. The culture was primarily composed of <i>Methylobacter marinus</i>, <i>Methylophaga marina</i>, and the microalga <i>Picochlorum oklahomensis</i>. Gas consumption, growth, and ectoine production were evaluated under varying salinity levels and osmotic stress. The NaCl concentrations above 6% negatively impacted CH<sub>4</sub> oxidation and inhibited ectoine synthesis, while osmotic shocks enhanced ectoine accumulation, with a maximum ectoine content of 51.3 mg<sub>ectoine</sub> g<sub>VSS</sub><sup>−1</sup>&nbsp;at 4.5% NaCl. This study is the first to report ectoine production from methanotroph-microalgal cultures, showing its potential for biogas valorization into high-value bio-based chemicals, like ectoine, marking a significant step toward sustainable biogas utilization.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Ectoine production through a marine methanotroph-microalgae culture allows complete biogas valorization","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:33:36.991798","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ectoine; GHG; Methanotrophs; Microalgae","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-09-18","DateCreate":"2025-05-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001408328000001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jenvman.2025.124223"},"refs":null,"anarec":{"AnaID":407945,"PubliDate":2025,"Pagination":"124223","XtraPublOfAnaID":null,"ISBN":null,"Volume":"375","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43053,"SerRR":"Journal of Environmental Management. 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