{"refrec":{"BRefID":409070,"RR":"<b>Lee, C.E.; Messer, L.F.; Wattiez, R.; Matallana-Surget, S.</b> (2025). Decoding microbial plastic colonisation: Multi‐omic insights into the fast‐evolving dynamics of early‐stage biofilms. <i>Proteomics (Weinh., Print) 25(7)</i>: e202400208. <a href=\"https://dx.doi.org/10.1002/pmic.202400208\" target=\"_blank\">https://dx.doi.org/10.1002/pmic.202400208</a>","BEntID":406873,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Proteomics 25(7)</i>: e202400208. <a href=\"https://dx.doi.org/10.1002/pmic.202400208\" target=\"_blank\">https://dx.doi.org/10.1002/pmic.202400208</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lee, C.E.; Messer, L.F.; Wattiez, R.; Matallana-Surget, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lee, C.E. <i>et al.</i>","Englishabstract":"Marine plastispheres represent dynamic microhabitats where microorganisms colonise plastic debris and interact. Metaproteomics has provided novel insights into the metabolic processes within these communities; however, the early metabolic interactions driving the plastisphere formation remain unclear. This study utilised metaproteomic and metagenomic approaches to explore early plastisphere formation on low-density polyethylene (LDPE) over 3 (D3) and 7 (D7) days, focusing on microbial diversity, activity and biofilm development. In total, 2948 proteins were analysed, revealing dominant proteomes from <i>Pseudomonas</i> and <i>Marinomonas</i>, with near-complete metagenome-assembled genomes (MAGs). <i>Pseudomonas</i> dominated at D3, whilst at D7, <i>Marinomonas</i>, along with <i>Acinetobacter, Vibrio</i> and other genera became more prevalent. <i>Pseudomonas</i> and <i>Marinomonas</i> showed high expression of reactive oxygen species (ROS) suppression proteins, associated with oxidative stress regulation, whilst granule formation, and alternative carbon utilisation enzymes, also indicated nutrient limitations. Interestingly, 13 alkanes and other xenobiotic degradation enzymes were expressed by five genera. The expression of toxins, several type VI secretion system (TVISS) proteins, and biofilm formation proteins by <i>Pseudomonas</i> indicated their competitive advantage against other taxa. Upregulated metabolic pathways relating to substrate transport also suggested enhanced nutrient cross-feeding within the more diverse biofilm community. These insights enhance our understanding of plastisphere ecology and its potential for biotechnological applications","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Decoding microbial plastic colonisation: Multi‐omic insights into the fast‐evolving dynamics of early‐stage biofilms","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-09-25 01:36:50.712317","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":" biofilm formation | marine biofilms | metaproteome | plastic debris | plastisphere","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-09-18","DateCreate":"2025-06-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001389917300001","VABBcode":null,"OpenAcc":1,"DOI":"10.1002/pmic.202400208"},"refs":null,"anarec":{"AnaID":409070,"PubliDate":2025,"Pagination":"e202400208","XtraPublOfAnaID":null,"ISBN":null,"Volume":"25","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":220678,"SerRR":"Proteomics. 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