{"refrec":{"BRefID":410233,"RR":"<b>Fachetti, B.S.; Turiel-Silva, M.; Wendt, C.; Costi, H.T.; da Silva, E.O.; Rodrigues, A.P.D.; de Souza, W.; Miranda, K.; Diniz, J.A.P.</b> (2025). 3D electron microscopy reveals the structural complexity of the intravacuolar membranous network in <i>Cyrilia lignieresi</i>‐infected erythrocytes of the fish <i>Synbranchus marmoratus</i>. <i>J. Eukaryot. Microbiol. 72(4)</i>: 1-11. <a href=\"https://dx.doi.org/10.1111/jeu.70031\" target=\"_blank\">https://dx.doi.org/10.1111/jeu.70031</a>","BEntID":408042,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>J. Eukaryot. Microbiol. 72(4)</i>: 1-11. <a href=\"https://dx.doi.org/10.1111/jeu.70031\" target=\"_blank\">https://dx.doi.org/10.1111/jeu.70031</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fachetti, B.S.; Turiel-Silva, M.; Wendt, C.; Costi, H.T.; da Silva, E.O.; Rodrigues, A.P.D.; de Souza, W.; Miranda, K.; Diniz, J.A.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fachetti, B.S. <i>et al.</i>","Englishabstract":"This study employs advanced three-dimensional electron microscopy techniques, including Transmission Electron Microscopy (TEM) tomography and freeze-fracture imaging via scanning electron microscopy (SEM), to investigate the ultrastructural organization of <i>Cyrilia lignieresi</i>-infected red blood cells (iRBCs) in the host fish <i>Synbranchus marmoratus</i>. The analysis focuses on the parasitophorous vacuole (PV) and reveals a highly complex intravacuolar membranous network (IVN) composed of vesicles, tubules, and interconnected membranous structures. These elements exhibit considerable diversity in size, morphology, and electron density, suggesting dynamic functional roles in the parasite–host interaction. The electron tomography and three-dimensional reconstructions data provide unprecedented insights into the spatial organization and potential functional significance of these membranous systems. 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