{"refrec":{"BRefID":411194,"RR":"<b>Mattelin, V.; Van Landuyt, J.; Kerkhof, F-M.; Minnebo, Y.; Boon, N.</b> (2025). Integrating taxonomic and phenotypic information through FISH-enhanced flow cytometry for microbial community dynamics analysis. <i>Methods and Protocols 13(8)</i>: e01973-24. <a href=\"https://dx.doi.org/10.1128/spectrum.01973-24\" target=\"_blank\">https://dx.doi.org/10.1128/spectrum.01973-24</a>","BEntID":409003,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Methods and Protocols 13(8)</i>: e01973-24. <a href=\"https://dx.doi.org/10.1128/spectrum.01973-24\" target=\"_blank\">https://dx.doi.org/10.1128/spectrum.01973-24</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Mattelin, V.; Van Landuyt, J.; Kerkhof, F-M.; Minnebo, Y.; Boon, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Mattelin, V. <i>et al.</i>","Englishabstract":"Flow cytometry is a powerful tool to monitor microbial communities, as it allows tracking both changes in the subpopulations and cell numbers at high throughput and a low sample cost. This information can be combined in a phenotypic fingerprint that can be leveraged for diversity analysis. However, as isogenic individuals can manifest phenotypic diversity, for example, due to differing physiological state and phenotypic plasticity, combining the phenotypic information with taxonomic information adds an extra dimension for describing the dynamics of a microbial community. In this research, taxonomic information was incorporated in the microbial fingerprint through fluorescent <i>in situ</i> hybridization (FISH) at a single-cell level. To validate this concept and explore its versatility, two ecosystems with different micro-biodiversity were considered. In the first environment, marine bacteria were monitored for plastic biodegradation in a trickling filter, and in the second, an <i>in vitro</i> simulated human gut microbiome was followed over time. Samples were prepared using different (staining) methods, including FISH, and beta diversity analysis was used to evaluate the level of distinction between differently treated groups in both environments. As a reference to correlate increased distinction with the incorporation of taxonomic information, 16S rRNA gene sequencing was used. Finally, a predictive algorithm was trained to correctly classify samples in the differently treated groups. The results showed that the implementation of FISH in flow cytometry provides more information on a single-cell level to answer specific scientific questions, like distinguishing between phenotypically similar communities or following a specific taxonomic group over time.<h3>IMPORTANCE</h3>Understanding microbial communities is crucial for elucidating their role in maintaining ecosystem health and stability. Researchers are increasingly interested in studying microbial communities by looking at not just their genetic makeup but also their physical traits and functions. In our study, we used common techniques like fluorescence <i>in situ</i> hybridization and flow cytometry, along with advanced data analysis, to better understand these communities. This combination allowed us to gather and use data more effectively, demonstrating that these easy-to-use methods, when paired with proper analysis, can enhance our understanding of changing microbial ecosystems.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Integrating taxonomic and phenotypic information through FISH-enhanced flow cytometry for microbial community dynamics analysis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-08-14 01:34:30.461736","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"phenotypic fingerprinting, fluorescence <i>in situ</i> hybridization, microbial community dynamics","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-08-07","DateCreate":"2025-08-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001513171100001","VABBcode":null,"OpenAcc":1,"DOI":"10.1128/spectrum.01973-24"},"refs":null,"anarec":{"AnaID":411194,"PubliDate":2025,"Pagination":"e01973-24","XtraPublOfAnaID":null,"ISBN":null,"Volume":"13","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":363970,"SerRR":"Methods and Protocols. 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