{"refrec":{"BRefID":418992,"RR":"<b>Simons, D.-L.; Hipperson, H.; Webb, T.; Spencer, M.; Horsburgh, G.J.; Mieszkowska, N.</b> (2025). Characterizing rocky intertidal biodiversity using environmental DNA metabarcoding from local to national scales. <i>Environmental DNA 7(5)</i>: e70203. <a href=\"https://dx.doi.org/10.1002/edn3.70203\" target=\"_blank\">https://dx.doi.org/10.1002/edn3.70203</a>","BEntID":416822,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>Environmental DNA 7(5)</i>: e70203. <a href=\"https://dx.doi.org/10.1002/edn3.70203\" target=\"_blank\">https://dx.doi.org/10.1002/edn3.70203</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Simons, D.-L.; Hipperson, H.; Webb, T.; Spencer, M.; Horsburgh, G.J.; Mieszkowska, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Simons, D.-L. <i>et al.</i>","Englishabstract":"Efficient and scalable methods for monitoring marine biodiversity are critical for understanding ecological change in coastal environments, given the limited resources available. Environmental DNA (eDNA) metabarcoding shows promise for monitoring coastal taxa, but its ability to differentiate communities from different locations remains insufficiently understood, particularly in dynamic marine environments. Here, we evaluate the effectiveness and resolution capacity of eDNA metabarcoding in detecting rocky intertidal taxa across three spatial scales—national, regional, and local—in the United Kingdom. Onshore surface-water samples were collected from 32 sites across five UK Regional Seas from rockpools in high and low shore zones, as well as directly from the sea. We detected 1026 target taxa within 442 families and 19 phyla using two established markers targeting invertebrates (COI) and macroalgae (18S). Distinct eDNA signals were found at all spatial scales, indicating local discreteness even between vertical shore heights within the same sites. Communities were more discrete at larger scales (i.e., between regions) than at smaller scales (i.e., between shore heights). eDNA signals were more strongly structured by geographical location than by vertical shore height as a probable consequence of greater DNA homogenization over the tidal cycle at smaller spatial scales. Established ecological zonation patterns were reflected in eDNA signals, with higher richness at lower shore heights, reflecting abiotic stress gradients. Detections of cold-affinity boreal species increased with latitude, while warm-affinity lusitanian species declined with latitude. Our work supports the utility of eDNA metabarcoding for multiscale biodiversity monitoring in dynamic marine environments and for detections beyond this study's target taxa. We recommend the adoption of scale-appropriate sampling protocols to optimize the benefits of eDNA, such as prioritizing open water sampling at high tide for broad-scale assessments and rockpool sampling at low tide for capturing local-scale patterns. Future work should validate detections through direct visual comparisons.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterizing rocky intertidal biodiversity using environmental DNA metabarcoding from local to national scales","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-10-23 01:36:34.346850","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":" biodiversity monitoring; coastal ecosystems;  invertebrates; macroalgae; marine eDNA; spatial ecology","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-10-16","DateCreate":"2025-10-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1002/edn3.70203"},"refs":null,"anarec":{"AnaID":418992,"PubliDate":2025,"Pagination":"e70203","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":314269,"SerRR":"Environmental DNA. 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