{"refrec":{"BRefID":4226,"RR":"<b>Nakamura, M.; Kobayashi, T.; Yoshiura, Y.; Nagahama, Y.</b> (2000). Role of endogenous steroid hormones on gonadal sex differentiation in fish, <b><i>in</i></b>: Norberg, B. <i>et al.</i> <i>Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999.</i> pp. 247-249","BEntID":4226,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Norberg, B. <i>et al.</i> <i>Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish,</i> 6: pp. 247-249","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nakamura, M.; Kobayashi, T.; Yoshiura, Y.; Nagahama, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nakamura, M. <i>et al.</i>","Englishabstract":"To clarify the role endogenous estrogen plays in sex differentiation in fish, we examined the effects of aromatase inhibitor (AI) on genetically controlled, all-female tilapia. Three different treatment protocols caused fish to develop testes: AI at 200 µg/g diet, AI at 500 µg/g diet, and methyltestosterone (MT) from 8 days after hatching (DAH) for 22 days. In contrast, all fish that received a diet containing both AI 500 and estradiol-17 beta 250 µg/g had normal ovaries. Therefore, we conclude that AI effectively masculinizes genetic females. A corollary conclusion is that endogenous estrogen plays an important role in ovarian differentiation in tilapia. We also established immunohistochemically the presence of aromatase (arom) and 3 beta -hydroxysteroid dehydrogenase (3 beta -HSD) in the gonads of fish. Immunopositive cells for arom-antibody (AB) were present in the testes of fish treated with AI, but not in the testes of fish treated with MT. In contrast, positive cells for 3 beta -HSD were present in the testes of specimens treated with either AI or MT. These differences suggest that the mechanisms by which AI and MT affect the differentiation of steroid-producing cells and the expression of aromatase are different, despite both AI and MT being powerful masculinizing agents.","AbstractOtherLang":null,"BibLvlCode":"AMS","StandardTitle":"Role of endogenous steroid hormones on gonadal sex differentiation in fish","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-08 01:30:35.064653","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2000,"MonPub":null,"DateUpdate":"2020-11-09","DateCreate":"2000-11-07","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":4226,"PubliDate":2000,"Pagination":"247-249","XtraPublOfAnaID":null,"ISBN":"82-7461-048-2","Volume":"6","Issue":null,"BRefMon":4111,"BRefMonRR":"<b>Norberg, B. <i>et al.</i></b> (2000). 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