{"refrec":{"BRefID":4234,"RR":"<b>Kobayashi, T.; Kajiura-Kobayashi, H.; Nagahama, Y.</b> (2000). Gonadal formation and differentiation in a teleost, tilapia <i>Oreochromis niloticus</i>, <b><i>in</i></b>: Norberg, B. <i>et al.</i> (Ed.) <i>Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999.</i> pp. 259","BEntID":4234,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":", <b><i>in</i></b>: Norberg, B. <i>et al.</i> <i>Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish,</i> 6: pp. 259","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kobayashi, T.; Kajiura-Kobayashi, H.; Nagahama, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kobayashi, T.; Kajiura-Kobayashi, H.; Nagahama, Y.","Englishabstract":"To clarify the characteristics of germ cells during differentiation and gametogenesis, we cloned tilapia vasa homologue (vas) cDNA. Using vas, we examined the expression of vas in germ cell stages from PGCs to gametogenesis. <i>in situ</i> hybridization for vas mRNA indicated that signals are present in migrated PGCs at 0 dah. PGCs continued to express vas mRNA after incorporation in the gonadal anlage. During oogenesis, vas transcripts were expressed strongly in stages from oogonia to diplotene oocytes. 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