{"refrec":{"BRefID":435608,"RR":"<b>Ganzha, E.V.; Dien, T.D.; Pavlov, E.D.</b> (2025). Floating microplastics with biofilm changes feeding behavior of climbing perch<i> Anabas testudineus</i>. <i>Microplastics  4(3)</i>: 62. <a href=\"https://dx.doi.org/10.3390/microplastics4030062\" target=\"_blank\">https://dx.doi.org/10.3390/microplastics4030062</a>","BEntID":433438,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Microplastics  4(3)</i>: 62. <a href=\"https://dx.doi.org/10.3390/microplastics4030062\" target=\"_blank\">https://dx.doi.org/10.3390/microplastics4030062</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ganzha, E.V.; Dien, T.D.; Pavlov, E.D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ganzha, E.V.; Dien, T.D.; Pavlov, E.D.","Englishabstract":"The climbing perch, Anabas testudineus, is one of the most widely distributed freshwater amphibious fishes in South and Southeast Asia, inhabiting both natural and artificial water bodies polluted by plastic waste. Current mesocosm experimental study aimed to investigate behavioral responses of wild fish to floating expanded polystyrene (EPS) pellets, with a focus on the biofilm developing on their surface. For biofilm formation, the pellets (diameter 3–4 mm) were exposed for two, six, and fourteen days in an irrigation canal inhabited by climbing perch. Development of an intensive biofilm was observed on days 6 and 14 of exposure, characterized by a high diversity of organisms, including protozoa, cyanobacteria, algae, amoebae, and fungi. Fish feeding behavior was observed in the presence of feed pellets, clean EPS pellets, and three variants of EPS pellets with biofilm developed on their surfaces in the freshwater environment. The fish rapidly grasped and ingested feed pellets compared to all variants of plastic pellets. Climbing perch grasped all types of EPS pellets but always rejected them after oral cavity testing. The time to the first grasp was significantly longer for both clean EPS and EPS exposed for two days compared to feed pellets. Biofilm appeared to function as a taste deterrent for the fish: the duration of oral cavity testing was negatively correlated with the EPS pellet exposure timings in natural conditions. We suggest that floating plastic stimulates foraging behavior in the fish, and the duration of this behavior was significantly longer than that observed with feed pellets. The similarity of positive buoyant EPS pellets to natural food objects may stimulate the fish movements towards the water surface, which likely results in greater energy expenditure and increased risk of predation, without any apparent benefit to the individual.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Floating microplastics with biofilm changes feeding behavior of climbing perch<i> Anabas testudineus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-10-31 01:34:51.853418","timezone_type":1,"timezone":"+01:00"},"AuthorKeywords":"expanded polystyrene; biofilm; foraging behaviour; taste stimuli; natural environments; mesocosm experiments; tropical freshwater bodies","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-10-24","DateCreate":"2025-10-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.3390/microplastics4030062"},"refs":null,"anarec":{"AnaID":435608,"PubliDate":2025,"Pagination":"62","XtraPublOfAnaID":null,"ISBN":null,"Volume":"4","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":416087,"SerRR":"Microplastics . 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