{"refrec":{"BRefID":436632,"RR":"<b>Alves, C.; Baptista, L.; Madeira, P.; Vaz Álvaro, N.; Santos, A.M.; Meimberg, H.; Ávila, S.P.; Curto, M.</b> (2025). Mind the gap: DNA barcoding on Pachygrapsus crabs. <i>Metabarcoding and Metagenomics 9</i>: 297–313. <a href=\"https://dx.doi.org/10.3897/mbmg.9.152554\" target=\"_blank\">https://dx.doi.org/10.3897/mbmg.9.152554</a>","BEntID":434462,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>Metabarcoding and Metagenomics 9</i>: 297–313. <a href=\"https://dx.doi.org/10.3897/mbmg.9.152554\" target=\"_blank\">https://dx.doi.org/10.3897/mbmg.9.152554</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Alves, C.; Baptista, L.; Madeira, P.; Vaz Álvaro, N.; Santos, A.M.; Meimberg, H.; Ávila, S.P.; Curto, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Alves, C. <i>et al.</i>","Englishabstract":"<span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">DNA barcode databases for marine invertebrates faces significant challenges, with several species revealing taxonomic issues like misidentification, ambiguity or shared barcodes. This highlights the importance of the availability of robust genetic data to resolve taxonomic uncertainties and improve species identification, which in many groups of invertebrates, like crustaceans, is lacking. Based on molecular data from the Cytochrome </span><i>c</i><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> oxidase I 5′ gene region (</span><i>COI</i><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">) for barcoding, this study highlights some of these challenges by addressing existing taxonomic ambiguities and highlighting the scarcity of genetic data for several of the shore crab genus </span><span style=\"color:rgb(0,102,0);\"><i>Pachygrapsus</i></span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> Randall, 1840. As expected, molecular data availability is highly skewed towards taxa more prevalent in the Northern Hemisphere. Our analysis reveals a clear barcoding gap amongst </span><span style=\"color:rgb(0,102,0);\"><i>Pachygrapsus</i></span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> species, with average interspecific </span><i>p</i><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">-distances of 18.75%, exceeding previously reported values for crustaceans. Furthermore, high intraspecific variation (0.70% average) suggests potential taxonomic issues, particularly within </span><span style=\"color:rgb(0,102,0);\"><i>P. gracilis</i></span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> (de Saussure, 1857). East and west Atlantic </span><span style=\"color:rgb(0,102,0);\"><i>P. gracilis</i></span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> populations exhibit substantial divergence (3.6% </span><i>p</i><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">-distance), raising the possibility of ongoing speciation due to limited gene flow across the Atlantic Ocean. Similarly, populations of </span><span style=\"color:rgb(0,102,0);\"><i>P. crassipes</i></span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> in the east and west North Pacific exhibit substantial divergence (3.3% </span><i>p</i><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">-distance), which suggests possibly another speciation process. </span><i>COI</i><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> barcoding proves effective for species identification within </span><span style=\"color:rgb(0,102,0);\"><i>Pachygrapsus</i></span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\"> and in unravelling intraspecific patterns indicative of cryptic diversity guiding further taxonomic revisions integrating morphological, genetic and ecological data.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Mind the gap: DNA barcoding on Pachygrapsus crabs","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-12-23 01:33:19.238537","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">Barcode; COI; </span><span style=\"background-color:rgb(255,255,255);color:rgb(0,102,0);\">Crustacea</span><span style=\"background-color:rgb(255,255,255);color:rgb(51,51,51);\">; cryptic species; haplotype network; phylogeny</span>","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2025-12-16","DateCreate":"2025-12-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.3897/mbmg.9.152554"},"refs":null,"anarec":{"AnaID":436632,"PubliDate":2025,"Pagination":"297–313","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":328125,"SerRR":"Metabarcoding and Metagenomics. 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