{"refrec":{"BRefID":437380,"RR":"<b>Sorokin, D.Y.; Merkel, A.Y.; Bale, N.J.; Koenen, M.; Sinninghe Damsté, J.S; Marturano, L.; Messina, E.; La Cono, V.; Yakimov, M.M.</b> (2025). Natronomicrosphaera hydrolytica, gen. nov., sp. nov., a first representative of the phylum Planctomycetota from soda lakes. <i>Syst. Appl. Microbiol. 48(3)</i>: 126608. <a href=\"https://dx.doi.org/10.1016/j.syapm.2025.126608\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2025.126608</a>","BEntID":435211,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Syst. Appl. Microbiol. 48(3)</i>: 126608. <a href=\"https://dx.doi.org/10.1016/j.syapm.2025.126608\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2025.126608</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sorokin, D.Y.; Merkel, A.Y.; Bale, N.J.; Koenen, M.; Sinninghe Damsté, J.S; Marturano, L.; Messina, E.; La Cono, V.; Yakimov, M.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sorokin, D.Y. <i>et al.</i>","Englishabstract":"<span style=\"color:rgb(31,31,31);\">Despite intensive microbiological characterization of soda lake microbial communities, no culturable representatives from the phylum </span><i>Planctomycetota</i><span style=\"color:rgb(31,31,31);\"> have been isolated from these haloalkaline habitats. In the context of studying polysaccharide utilization by soda lake microbial communities, we used polysaccharide hyaluronic acid as enrichment substrate at aerobic, moderate haloalkaline conditions (1&nbsp;M total Na</span><sup>+</sup><span style=\"color:rgb(31,31,31);\">, pH&nbsp;9.5). This resulted in a selective enrichment and isolation in pure culture of a bacterial strain AB-hyl4 belonging to </span><i>Planctomycetota</i><span style=\"color:rgb(31,31,31);\">. The cells are tiny motile cocci growing in large aggregates, with the Gram-negative type of ultrastructure and producing a yellow pigment. This obligate aerobic saccharolytic heterotroph has an extremely narrow growth substrate range including, besides hyaluronic acid, melezitose and glycerol. The membrane lipids consist of phosphatidylcholine and two types of neutral lipids, including hopanoids and monounsaturated C17 and C19 hydrocarbons. Phylogenomic analysis placed the isolate into the family </span><i>Phycisphaeraceae</i><span style=\"color:rgb(31,31,31);\">, class </span><i>Phycisphaerae,</i><span style=\"color:rgb(31,31,31);\"> as a new genus-level lineage. Its genome contained a gene encoding a polysaccharide lyase from the PL8 family which is probably responsible for the degradation of hyaluronic acid to a dimer, followed by its transport and hydrolysis into monomers in periplasm and final glycolytic degradation in cytoplasm. On the basis of distinct phenotypic and genomic properties, strain AB-hyl4</span><sup>T</sup><span style=\"color:rgb(31,31,31);\"> (DSM 117794&nbsp;=&nbsp;UQM 41914) is proposed to be classified as </span><i>Natronomicrosphaera hydrolytica</i><span style=\"color:rgb(31,31,31);\"> gen. nov., sp. nov.</span>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Natronomicrosphaera hydrolytica, gen. nov., sp. nov., a first representative of the phylum Planctomycetota from soda lakes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-01 01:32:41.803707","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"<p style=\"margin-left:0px;\">Soda lakes; Hyaluronic acid; Haloalkaliphilic; <i>Planctomycetota; Phicisphaeraceae</i>","OtherDescriptors":null,"Notes":null,"AnaPub":2025,"MonPub":null,"DateUpdate":"2026-01-23","DateCreate":"2026-01-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.syapm.2025.126608"},"refs":null,"anarec":{"AnaID":437380,"PubliDate":2025,"Pagination":"126608","XtraPublOfAnaID":null,"ISBN":null,"Volume":"48","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43944,"SerRR":"Systematic and Applied Microbiology. 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