{"refrec":{"BRefID":437786,"RR":"<b>Liu, J.; Glukhov, E.; De Clerck, O.; Gerwick, W.H.; Donia, M.S.</b> (2026). Environmentally controlled production of pagoamide A in marine macroalgae by an intracellular bacterial symbiont. <i>Curr. Biol. 36(1)</i>: 63-79.E6. <a href=\"https://dx.doi.org/10.1016/j.cub.2025.11.023\" target=\"_blank\">https://dx.doi.org/10.1016/j.cub.2025.11.023</a>","BEntID":435617,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Curr. Biol. 36(1)</i>: 63-79.E6. <a href=\"https://dx.doi.org/10.1016/j.cub.2025.11.023\" target=\"_blank\">https://dx.doi.org/10.1016/j.cub.2025.11.023</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Liu, J.; Glukhov, E.; De Clerck, O.; Gerwick, W.H.; Donia, M.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Liu, J. <i>et al.</i>","Englishabstract":"Marine algae are a rich source of diverse molecules, most of which are thought to be produced by the alga itself. We recently reported the discovery of pagoamide A from a cultured marine macroalga collected from American Samoa. Here, we found that the production of pagoamide A is conditional upon environmental temperature. Using comparative metagenomic, metatranscriptomic, and metabolomic analyses of algal cultures, we identified a nonribosomal peptide synthetase biosynthetic gene cluster (NRPS BGC) in the algal microbiome that varies in abundance between producing and non-producing conditions and whose architecture and biosynthetic logic match pagoamide A (named <i>pag</i>). <i>pag</i> belongs to a bacterium that we named “<i>Candidatus</i> Bryopsidiphilus pagoamidifaciens BP1,” a new genus in the family Amoebophilaceae and a relative of amoeba, arthropod, and nematode endosymbionts. <i>Ca.</i> B. pagoamidifaciens lives intracellularly in its <i>Bryopsis</i> sp. algal host, harbors a reduced genome (1.7 Mbp), has lost most genes essential for free living, and is enriched in genes containing eukaryotic domains. By quantitatively monitoring longitudinal algal cultures under varying conditions for 9 weeks, we found that the abundance of both <i>Ca.</i> B. pagoamidifaciens and pagoamide A undergoes dramatic fluctuations in response to temperature changes. Finally, we discovered three additional strains of <i>Ca.</i> B. pagoamidifaciens that vary in their NRPS BGCs and eukaryotic domain-containing genes from algal samples of diverse geographical origins. Our findings suggest that symbiont-derived production of algal molecules is more common than previously anticipated and provide a unique case of environmental control of both symbiont and chemical levels in marine algae.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Environmentally controlled production of pagoamide A in marine macroalgae by an intracellular bacterial symbiont","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:33:05.762359","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2026,"MonPub":null,"DateUpdate":"2026-02-20","DateCreate":"2026-02-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001660286700001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.cub.2025.11.023"},"refs":null,"anarec":{"AnaID":437786,"PubliDate":2026,"Pagination":"63-79.E6","XtraPublOfAnaID":null,"ISBN":null,"Volume":"36","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":72005,"SerRR":"Current Biology. 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