{"refrec":{"BRefID":439243,"RR":"<b>Hewson, I.; Rede, J.</b> (2026). Investigating marine mass mortality using rapid nucleic acid sequencing and artificial intelligence, <b><i>in</i></b>: Byers, J.E. <i>et al.</i> <i>The ecology and evolution of marine parasites and disease. Ecology and evolution of infectious diseases series,</i> : pp. 15-36. <a href=\"https://dx.doi.org/10.1093/9780197790847.003.0003\" target=\"_blank\">https://dx.doi.org/10.1093/9780197790847.003.0003</a>","BEntID":437076,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Byers, J.E. <i>et al.</i> <i>The ecology and evolution of marine parasites and disease. Ecology and evolution of infectious diseases series,</i> : pp. 15-36. <a href=\"https://dx.doi.org/10.1093/9780197790847.003.0003\" target=\"_blank\">https://dx.doi.org/10.1093/9780197790847.003.0003</a>","DocTypID":17,"DocType":"Book chapters","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hewson, I.; Rede, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hewson, I.; Rede, J.","Englishabstract":"Mass mortality events are becoming more frequent in the Anthropocene due to heightened host stress, increased pathogen virulence, and other factors influencing pathogen–host interactions. At the same time, advances in diagnostic methods, particularly molecular approaches, have significantly improved the identification of candidate disease agents. However, the transition from traditional microscopy and culture-based methods to culture-independent approaches, together with their increased accessibility, introduces risks of misattribution since functional plasticity can lead to spurious associations with disease. This review begins with an overview of bacterial diversity, physiology, and pathogenesis and provides examples from marine disease phenomena. It then critically examines current and emerging methodologies for studying marine disease etiology, including microbial cultivation strategies, nucleic acid sequencing techniques, and artificial intelligence applications. 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