{"refrec":{"BRefID":58152,"RR":"<b>Siu, W.H.L.; Cao, J.; Jack, R.W.; Wu, R.S.S.; Richardson, B.J.; Xu, L.; Lam, P.K.S.</b> (2004). Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (<i>Perna viridis</i>). <i>Aquat. Toxicol. 66(4)</i>: 381-392. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2003.10.006\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2003.10.006</a>","BEntID":55786,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 66(4)</i>: 381-392. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2003.10.006\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2003.10.006</a>","DocTypID":9,"DocType":"Map","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Siu, W.H.L.; Cao, J.; Jack, R.W.; Wu, R.S.S.; Richardson, B.J.; Xu, L.; Lam, P.K.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Siu, W.H.L. <i>et al.</i>","Englishabstract":"Green-lipped mussels (<i>Perna viridis</i>) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30 µg l<ssup>-1</sup> for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing <i>P. viridis</i> hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (<i>Perna viridis</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:33:14.980000","timezone_type":1,"timezone":"+00:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"benzo[a]pyrene; haemocyte; DNA damage; micronucleus; Perna viridis;comet assay","OtherDescriptors":"Benzo[a]pyrene; Haemocyte; DNA damage; Micronucleus; Perna viridis; Comet assay","Notes":null,"AnaPub":2004,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2004-03-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000189228100004","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.aquatox.2003.10.006"},"refs":null,"anarec":{"AnaID":58152,"PubliDate":2004,"Pagination":"381-392","XtraPublOfAnaID":null,"ISBN":null,"Volume":"66","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":381,"Pages":12,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"City University of Hong Kong","Email":null,"Line2":null,"EnvName":"China"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Siu","Firstname":null,"Initials":"W.H.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":43478,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Cao","Firstname":null,"Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":43479,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Jack","Firstname":null,"Initials":"R.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":43480,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wu","Firstname":"Rudolf","Initials":"R.S.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":7748,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Richardson","Firstname":"Bruce","Initials":"B.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":7746,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Xu","Firstname":null,"Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":43483,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lam","Firstname":"Paul","Initials":"P.K.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55786,"AutID":7747,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":1,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":56157,"LibID":36,"BRefID":58152,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"56157","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTermPath":"Acids > Organic compounds > Organic acids > Nucleic acids > DNA"},{"ThesaurusTerm":"Haemocytes","ThestID":119059,"Acronym":"CAB","ThesTermPath":"Cells > Blood cells > Haemocytes"},{"ThesaurusTerm":"Marine molluscs","ThestID":5010,"Acronym":"ASFA","ThesTermPath":"Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine molluscs"},{"ThesaurusTerm":"Hong Kong","ThestID":183955,"Acronym":"ASFA_GEOT","ThesTermPath":"Hong Kong"}],"thestermsASFA":[{"ThesaurusTerm":"DNA"},{"ThesaurusTerm":"Haemocytes"},{"ThesaurusTerm":"Hong Kong"},{"ThesaurusTerm":"Marine molluscs"}],"taxtermsASFA":[{"TaxTerm":"Perna viridis"}],"geotermsASFA":[{"GeoTerm":"Hong Kong"}],"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":55786,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2003.10.006","externalID":"10.1016/j.aquatox.2003.10.006","URLTypeCode":"DOI","URLID":1987,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Haemocytes","ThestID":119059,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Hong Kong","ThestID":183955,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Marine molluscs","ThestID":5010,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Perna viridis","AphiaID":367822,"TaxtID":23394}],"geoterms":[{"GeoTerm":"Hong Kong","GeoObjectID":8759,"GeotID":2238}],"othterms":[{"OtherTerm":"Benzo[a]pyrene","OthtID":2305},{"OtherTerm":"Comet assay","OthtID":2602},{"OtherTerm":"Dna damage","OthtID":7878},{"OtherTerm":"Micronucleus","OthtID":9971}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"DNA","DutchTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Haemocytes","DutchTerm":null,"ThestID":119059,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Hong Kong","DutchTerm":null,"ThestID":183955,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Marine molluscs","DutchTerm":"Mariene weekdieren","ThestID":5010,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Perna viridis","DutchTerm":null,"AphiaID":367822,"TaxtID":23394}],"geotermsFRIS":[{"GeoTerm":"Hong Kong","DutchTerm":null}],"othtermsFRIS":[{"OtherTerm":"Benzo[a]pyrene","DutchTerm":null,"OthtID":2305},{"OtherTerm":"Comet assay","DutchTerm":null,"OthtID":2602},{"OtherTerm":"Dna damage","DutchTerm":null,"OthtID":7878},{"OtherTerm":"Micronucleus","DutchTerm":null,"OthtID":9971}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2004-03-09 09:20:30.523000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-05-17 21:28:56.790000","timezone_type":3,"timezone":"Europe/Brussels"}}}