{"refrec":{"BRefID":58370,"RR":"<b>Lin, C.-H.; John, J.A.C.; Ou, L.W.; Chen, J.-C.; Lin, C.-H.; Chang, C.-Y.</b> (2004). Cloning and characterization of metallothionein gene in ayu <i>Plecoglossus altivelis</i>. <i>Aquat. Toxicol. 66(2)</i>: 111-124. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2003.06.003\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2003.06.003</a>","BEntID":55970,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 66(2)</i>: 111-124. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2003.06.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2003.06.003</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lin, C.-H.; John, J.A.C.; Ou, L.W.; Chen, J.-C.; Lin, C.-H.; Chang, C.-Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lin, C.-H. <i>et al.</i>","Englishabstract":"Metallothionein (MT) has been used widely as a potential molecular marker to detect the deleterious effects of heavy metals in aquatic ecosystem. Here we exposed ayu, <i>Plecoglossus altiveli</i>o zinc (Zn) and tested the distribution as well as the induction of MT in various tissues such as liver, kidney, intestine and stomach. MT induction was significant in liver tissue, followed by kidney and intestine, whereas no induction was detected in stomach. The gene encoding ayu MT was successfully cloned and characterized. Complete nucleotide sequencing and analysis of the 4.5 kb DNA fragment containing the ayu MT gene revealed that the gene has three exons interrupted by two introns, a 5'-flanking region of about 2.5 kb and about 1.6 kb of 3'-flanking region. In grouper heart and kidney cells, the 2.5 kb promoter containing eight metal responsive elements (MREs), two hepatic nuclear factor 5 responsive elements (HNF5REs) and one cAMP responsive element (CRE) had the highest reporter activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cloning and characterization of metallothionein gene in ayu <i>Plecoglossus altivelis</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ayu; cAMP responsive element (CRE); hepatic nuclear factor 5 responsiveelement (HNF5RE); metallothionein; metal responsive A element (MRE);promoter","OtherDescriptors":"Ayu; cAMP responsive element (CRE); Hepatic nuclear factor 5 responsive element (HNF5RE); Metallothionein; Metal responsive element (MRE); Promoter","Notes":null,"AnaPub":2004,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2004-03-11","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000220007000001","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.aquatox.2003.06.003"},"refs":null,"anarec":{"AnaID":58370,"PubliDate":2004,"Pagination":"111-124","XtraPublOfAnaID":null,"ISBN":null,"Volume":"66","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":111,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"336, Institute of Zoology (44)","Email":null,"Line2":null,"EnvName":"Taiwan, Province of China"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lin","Firstname":"Chih-Hung","Initials":"C.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55970,"AutID":43596,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"John","Firstname":"Joseph Abraham Christopher","Initials":"J.A.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55970,"AutID":43597,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ou","Firstname":"Li Wei","Initials":"L.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55970,"AutID":43598,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chen","Firstname":null,"Initials":"J.-C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55970,"AutID":18794,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lin","Firstname":"Cheng-Hui","Initials":"C.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55970,"AutID":43599,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chang","Firstname":"Chi-Yao","Initials":"C.-Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":55970,"AutID":43600,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":1,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":56380,"LibID":36,"BRefID":58370,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"56380","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Metallothioneins","ThestID":9559,"Acronym":"ASFA","ThesTermPath":"Chemical compounds > Organic compounds > Proteins > Metallothioneins"},{"ThesaurusTerm":"Metals","ThestID":5152,"Acronym":"ASFA","ThesTermPath":"Chemical elements > Metals"},{"ThesaurusTerm":"Ayu","ThestID":116456,"Acronym":"CAB","ThesTermPath":"Fishes > Osteichthyes > Salmoniformes > Plecoglossidae > Plecoglossus > Freshwater fishes > Ayu"},{"ThesaurusTerm":"Metallothionein","ThestID":153819,"Acronym":"CAB","ThesTermPath":"Peptides > Proteins > Sulfur proteins > Thioneins > Metalloproteins > Metallothionein"},{"ThesaurusTerm":"Promoters","ThestID":9586,"Acronym":"ASFA","ThesTermPath":"Promoters"}],"thestermsASFA":[{"ThesaurusTerm":"Ayu"},{"ThesaurusTerm":"Metallothionein"},{"ThesaurusTerm":"Metallothioneins"},{"ThesaurusTerm":"Metals"},{"ThesaurusTerm":"Promoters"}],"taxtermsASFA":[{"TaxTerm":"Plecoglossus altivelis"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":55970,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2003.06.003","externalID":"10.1016/j.aquatox.2003.06.003","URLTypeCode":"DOI","URLID":2022,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Ayu","ThestID":116456,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Metallothionein","ThestID":153819,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Metallothioneins","ThestID":9559,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Metals","ThestID":5152,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Promoters","ThestID":9586,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Plecoglossus altivelis [Ayu sweetfish]","AphiaID":293715,"TaxtID":22756}],"geoterms":null,"othterms":[{"OtherTerm":"Camp responsive element (cre)","OthtID":5336},{"OtherTerm":"Hepatic nuclear factor 5 responsive element (hnf5re)","OthtID":8168},{"OtherTerm":"Metal responsive element (mre)","OthtID":10981},{"OtherTerm":"Promoter","OthtID":11740}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Ayu","DutchTerm":null,"ThestID":116456,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Metallothionein","DutchTerm":null,"ThestID":153819,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Metallothioneins","DutchTerm":null,"ThestID":9559,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Metals","DutchTerm":"Metalen","ThestID":5152,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Promoters","DutchTerm":null,"ThestID":9586,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Plecoglossus altivelis","DutchTerm":null,"AphiaID":293715,"TaxtID":22756}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Camp responsive element (cre)","DutchTerm":null,"OthtID":5336},{"OtherTerm":"Hepatic nuclear factor 5 responsive element (hnf5re)","DutchTerm":null,"OthtID":8168},{"OtherTerm":"Metal responsive element (mre)","DutchTerm":null,"OthtID":10981},{"OtherTerm":"Promoter","DutchTerm":null,"OthtID":11740}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2004-03-11 14:23:41.723000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-05-17 21:28:56.790000","timezone_type":3,"timezone":"Europe/Brussels"}}}