{"refrec":{"BRefID":58788,"RR":"<b>Lacroix, A.; Hontela, A.</b> (2004). A comparative assessment of the adrenotoxic effects of cadmium in two teleost species, rainbow trout, <i>Oncorhynchus mykiss</i>, and yellow perch, <i>Perca flavescens</i>. <i>Aquat. Toxicol. 67(1)</i>: 13-21. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2003.11.010\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2003.11.010</a>","BEntID":56360,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 67(1)</i>: 13-21. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2003.11.010\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2003.11.010</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Lacroix, A.; Hontela, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lacroix, A.; Hontela, A.","Englishabstract":"Rainbow trout (<i>Oncorhynchus mykiss</i>) and yellow perch (<i>Perca flavescens</i>) have a different sensitivity to cadmium (Cd) in vivo (trout<LC50<perch). Metals and particularly Cd impair cortisol secretion by adrenocortical cells in both species. The purpose of the present study was to assess in vitro the effect of Cd on cortisol secretion by adrenocortical cells of trout and perch, to compare the sensitivity of adrenal steroidogenesis in these two teleosts. Adrenocortical cells were exposed to Cd for 60 min, then stimulated with ACTH, dbcAMP or with pregnenolone, a cortisol precursor. Cd inhibited ACTH-stimulated cortisol secretion in a dose-dependent manner in both fish species, however, the EC50s (concentration resulting in 50% inhibition of cortisol secretion) was significantly lower in trout (EC50=0.09 mM) than perch (EC50=0.26 mM). To test the specificity of Cd to act as an endocrine disrupter, the LC50 (concentration that kills 50% of the cells) was also evaluated to determine the LC50/EC50 ratio (LC50/EC50<sub>O.mykiss</sub> = 175.6>LC50/EC50<sub>P.flavescens</sub> = 37.7). Adrenocortical cells of trout were more sensitive than those of perch and Cd had a higher endocrine-disrupting potential and specificity in trout than in perch. However, in both species, Cd had the same effect on ACTH, dbcAMP and pregnenolone-stimulated cortisol secretion, with pregnenolone maintaining cortisol secretion until cell viability was impaired. These results confirm that for both species, Cd interferes in the signalling pathway of cortisol synthesis in a step prior to the pregnenolone formation. Data provided by the present study revealed important differences in vulnerability of adrenal steroidogenesis between rainbow trout and yellow perch.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A comparative assessment of the adrenotoxic effects of cadmium in two teleost species, rainbow trout, <i>Oncorhynchus mykiss</i>, and yellow perch, <i>Perca flavescens</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"fish; metals; endocrine toxicity; in vitro; cortisol; signallingpathways","OtherDescriptors":"Fish; Metals; Endocrine toxicity; In vitro; Cortisol; Signalling pathways","Notes":null,"AnaPub":2004,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2004-03-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000220264200002","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.aquatox.2003.11.010"},"refs":null,"anarec":{"AnaID":58788,"PubliDate":2004,"Pagination":"13-21","XtraPublOfAnaID":null,"ISBN":null,"Volume":"67","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":13,"Pages":9,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"4401, University Dr.","Email":null,"Line2":null,"EnvName":"Canada"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lacroix","Firstname":"Alexandra","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":56360,"AutID":43874,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hontela","Firstname":"Alice","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":56360,"AutID":2087,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":1,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":56888,"LibID":36,"BRefID":58788,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"56888","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; 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