{"refrec":{"BRefID":58791,"RR":"<b>Machella, N.; Regoli, F.; Cambria, A.; Santella, R.M.</b> (2004). Application of an immunoperoxidase staining method for detection of 7,8-dihydro-8-oxodeoxyguanosine as a biomarker of chemical-induced oxidative stress in marine organisms. <i>Aquat. Toxicol. 67(1)</i>: 23-32. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2003.11.008\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2003.11.008</a>","BEntID":56362,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 67(1)</i>: 23-32. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2003.11.008\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2003.11.008</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Machella, N.; Regoli, F.; Cambria, A.; Santella, R.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Machella, N. <i>et al.</i>","Englishabstract":"7,8-Dihydro-8-oxodeoxyguanosine (8-oxo-dG) is a typical modification of DNA caused by oxygen free radicals and can be an useful biomarker for pollutants inducing oxidative stress. An immunoperoxidase method using monoclonal antibody 1F7 toward 8-oxo-dG was applied to tissues and smeared cells of marine organisms for detection and quantification of oxidative DNA damage in such models. The assay, previously employed on human cells, was assessed for the first time on Mediterranean mussels (<i>Mytilus galloprovincialis</i>) and European eels (<i>Anguilla anguilla</i>), exposed to model pro-oxidant chemicals, namely benzo[a]pyrene (B[a]P) and copper. Quantification of 8-oxo-dG was microscopically carried out and expressed as relative nuclear staining intensity. Higher levels of oxidative DNA damage were detected in the digestive glands of treated mussels compared to controls, while the effect was less pronounced in haemocytes, characterized by more elevated basal levels of 8-oxo-dG. The assay was suitable for detection of 8-oxo-dG also in fish liver sections indicating consistent damage after B[a]P exposure. The main advantage of the immunohistochemical approach is the elimination of DNA extraction which considerably reduces the processing of biological samples. In addition, the assay requires small amounts of frozen tissues or fixed cells for detection of 8-oxo-dG and is potentially able to discriminate variable susceptibility to oxidative stress in different cell types. Although further investigations are required for the improvement and the validation of the assay in field conditions, laboratory exposures provided useful indications on the consistency of the approach and the efficacy of antibody 1F7 in marine organisms for a rapid assessment of pollutant-induced oxidative DNA damage.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Application of an immunoperoxidase staining method for detection of 7,8-dihydro-8-oxodeoxyguanosine as a biomarker of chemical-induced oxidative stress in marine organisms","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:33:14.980000","timezone_type":1,"timezone":"+00:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"7,8-dihydro-8-oxodeoxyguanosine; oxidative DNA damage; immunoperoxidasestaining; monoclonal antibody 1F7; mussels; eels","OtherDescriptors":"7,8-Dihydro-8-oxodeoxyguanosine; Oxidative DNA damage; Immunoperoxidase staining; Monoclonal antibody 1F7; Mussels; Eels","Notes":null,"AnaPub":2004,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2004-03-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000220264200003","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.aquatox.2003.11.008"},"refs":null,"anarec":{"AnaID":58791,"PubliDate":2004,"Pagination":"23-32","XtraPublOfAnaID":null,"ISBN":null,"Volume":"67","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":23,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Via Ranieri 65","Email":null,"Line2":null,"EnvName":"Italy"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Machella","Firstname":"Nicola","Initials":"N.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":56362,"AutID":43877,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":1,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Regoli","Firstname":null,"Initials":"F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":56362,"AutID":31150,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Cambria","Firstname":"Antonio","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":56362,"AutID":43878,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Santella","Firstname":"Regina","Initials":"R.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":56362,"AutID":43879,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":56892,"LibID":36,"BRefID":58791,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"56892","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTermPath":"Acids > Organic compounds > Organic acids > Nucleic acids > DNA"},{"ThesaurusTerm":"Marine organisms","ThestID":5011,"Acronym":"ASFA","ThesTermPath":"Aquatic organisms > Marine organisms"},{"ThesaurusTerm":"Oxidation","ThestID":5919,"Acronym":"ASFA","ThesTermPath":"Chemical reactions > Oxidation"},{"ThesaurusTerm":"Eels","ThestID":85220,"Acronym":"CSA","ThesTermPath":"Eels"},{"ThesaurusTerm":"Mussels","ThestID":154799,"Acronym":"CAB","ThesTermPath":"Eukaryotes > Animals > Invertebrates > Mollusca > Bivalvia > Shellfish > Mussels"},{"ThesaurusTerm":"Monoclonal antibodies","ThestID":9565,"Acronym":"ASFA","ThesTermPath":"Fluids > Body fluids > Serum > Antibodies > Monoclonal antibodies"},{"ThesaurusTerm":"MED","ThestID":185814,"Acronym":"ASFA_GEOT","ThesTermPath":"MED"},{"ThesaurusTerm":"Mussels","ThestID":69370,"Acronym":"CSA","ThesTermPath":"Mussels"},{"ThesaurusTerm":"Eels","ThestID":111071,"Acronym":"CAB","ThesTermPath":"Organisms > Aquatic organisms > Animals > Aquatic animals > Marine animals > Fishes > Aquatic animals > Marine fishes > Osteichthyes > Eels"}],"thestermsASFA":[{"ThesaurusTerm":"DNA"},{"ThesaurusTerm":"Eels"},{"ThesaurusTerm":"Eels"},{"ThesaurusTerm":"Marine organisms"},{"ThesaurusTerm":"MED"},{"ThesaurusTerm":"Monoclonal antibodies"},{"ThesaurusTerm":"Mussels"},{"ThesaurusTerm":"Mussels"},{"ThesaurusTerm":"Oxidation"}],"taxtermsASFA":[{"TaxTerm":"Anguilla anguilla (Linnaeus, 1758)"},{"TaxTerm":"Mytilus galloprovincialis Lamarck, 1819"}],"geotermsASFA":[{"GeoTerm":"Mediterranean Sea"}],"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":56362,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2003.11.008","externalID":"10.1016/j.aquatox.2003.11.008","URLTypeCode":"DOI","URLID":2116,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Eels","ThestID":85220,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Eels","ThestID":111071,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Marine organisms","ThestID":5011,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"MED","ThestID":185814,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Monoclonal antibodies","ThestID":9565,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mussels","ThestID":69370,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Mussels","ThestID":154799,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Oxidation","ThestID":5919,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Anguilla anguilla (Linnaeus, 1758)","AphiaID":126281,"TaxtID":16735},{"TaxTerm":"Mytilus galloprovincialis Lamarck, 1819 [Mediterranean mussel]","AphiaID":140481,"TaxtID":18296}],"geoterms":[{"GeoTerm":"Mediterranean Sea","GeoObjectID":1905,"GeotID":4101}],"othterms":[{"OtherTerm":"7,8-dihydro-8-oxodeoxyguanosine","OthtID":2020},{"OtherTerm":"Immunoperoxidase staining","OthtID":8236},{"OtherTerm":"Monoclonal antibody 1f7","OthtID":10001},{"OtherTerm":"Oxidative dna damage","OthtID":6628}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"DNA","DutchTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Eels","DutchTerm":"Paling","ThestID":85220,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Eels","DutchTerm":"Paling","ThestID":111071,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Marine organisms","DutchTerm":"Mariene organismen","ThestID":5011,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"MED","DutchTerm":null,"ThestID":185814,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Monoclonal antibodies","DutchTerm":null,"ThestID":9565,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mussels","DutchTerm":null,"ThestID":69370,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Mussels","DutchTerm":null,"ThestID":154799,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Oxidation","DutchTerm":"Oxidatie","ThestID":5919,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Anguilla anguilla (Linnaeus, 1758)","DutchTerm":"Paling","AphiaID":126281,"TaxtID":16735},{"TaxTerm":"Mytilus galloprovincialis Lamarck, 1819","DutchTerm":"Diepwatermossel","AphiaID":140481,"TaxtID":18296}],"geotermsFRIS":[{"GeoTerm":"Mediterranean Sea","DutchTerm":"MED"}],"othtermsFRIS":[{"OtherTerm":"7,8-dihydro-8-oxodeoxyguanosine","DutchTerm":null,"OthtID":2020},{"OtherTerm":"Immunoperoxidase staining","DutchTerm":null,"OthtID":8236},{"OtherTerm":"Monoclonal antibody 1f7","DutchTerm":null,"OthtID":10001},{"OtherTerm":"Oxidative dna damage","DutchTerm":null,"OthtID":6628}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2004-03-25 12:47:05.283000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-05-17 21:28:56.790000","timezone_type":3,"timezone":"Europe/Brussels"}}}