{"refrec":{"BRefID":59087,"RR":"<b>Koce, J.D.; Vilhar, B.; Bohanec, B.; Dermastia, M.</b> (2003). Genome size of Adriatic seagrasses. <i>Aquat. Bot. 77(1)</i>: 17-25. <a href=\"http://dx.doi.org/10.1016/s0304-3770(03)00072-x\" target=\"_blank\">http://dx.doi.org/10.1016/s0304-3770(03)00072-x</a>","BEntID":56683,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Bot. 77(1)</i>: 17-25. <a href=\"https://dx.doi.org/10.1016/s0304-3770(03)00072-x\" target=\"_blank\">https://dx.doi.org/10.1016/s0304-3770(03)00072-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Koce, J.D.; Vilhar, B.; Bohanec, B.; Dermastia, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Koce, J.D. <i>et al.</i>","Englishabstract":"Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for <i>Zostera noltii</i> (2n=12), 1.2 pg for <i>Zostera marina</i> (2n=12), 1.1 pg for <i>Cymodocea nodosa</i> (2n=28) and 6.2 pg for <i>Posidonia oceanica</i>, using <i>Pisum sativum</i> (2C-value=8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 °C. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genome size of Adriatic seagrasses","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:33:14.980000","timezone_type":1,"timezone":"+00:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"seagrasses; Alismatidae; genome size; C-value; DNA image cytometry","OtherDescriptors":null,"Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2004-04-02","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000185152700002","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/s0304-3770(03)00072-x"},"refs":null,"anarec":{"AnaID":59087,"PubliDate":2003,"Pagination":"17-25","XtraPublOfAnaID":null,"ISBN":null,"Volume":"77","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42195,"SerRR":"Aquatic Botany. 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