Document of bibliographic reference 59332

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Spatiotemporal patterning of reactive oxygen production and Ca²⁺ wave propagation in Fucus rhizoid cells

Abstract

Both Ca²⁺and reactive oxygen species (ROS) play critical signaling roles in plant responses to biotic and abiotic stress.However, the positioning of Ca²⁺and ROS (in particular H₂O₂) after a stress stimulus and their subcellular interactionsare poorly understood. Moreover, although information can be encoded in different patterns of cellular Ca²⁺ signals, little is known about the subcellular spatiotemporal patterns of ROS production or their significance for downstream responses. Here, we show that ROS production in response to hyperosmotic stress in embryonic cells of the alga Fucusserratusconsists of two distinct components. The first ROS component coincides closely with the origin of a Ca²⁺wave in the peripheral cytosol at the growing cell apex, has an extracellular origin, and is necessary for the Ca²⁺wave.Patch-clamp experiments show that a nonselective cation channel is stimulated by H₂O₂and may underlie the initialcytosolic Ca²⁺increase. Thus, the spatiotemporal pattern of the Ca²⁺wave is determined by peripheral ROS production.The second, later ROS component localizes to the mitochondria and is a direct consequence of the Ca²⁺ wave.The first component, but not the second, is required for short-term adaptation to hyperosmotic stress. Our resultshighlight the role of ROS in the patterning of a Ca²⁺signal in addition to its function in regulating cell wall strength inthe Fucus embryo.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000178740100006

Bibliographic citation

Coelho, S.M.; Taylor, A.R.; Ryan, K.P.; Sousa-Pinto, I.; Brown, M.T.; Brownlee, C. (2002). Spatiotemporal patterning of reactive oxygen production and Ca²⁺ wave propagation in Fucus rhizoid cells. Plant Cell 14: 2369-2381. https://dx.doi.org/10.1105/tpc.003285

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true